短小芽孢杆菌(Bacillus pumilus)X93胞外产物对病原弧菌抑制效果的研究  

Inhibition of extracellular products of Bacillus pumilusX93 on pathogenic vibrio

作　　者：王娟[1] 封永辉 张庆华[3] 

机构地区：[1]阜阳师范学院生命科学学院,安徽阜阳236037 [2]上海浩思海洋生物科技有限公司,上海201204 [3]上海海洋大学水产与生命学院,上海201306

出　　处：《阜阳师范学院学报（自然科学版）》2013年第1期31-35,共5页Journal of Fuyang Normal University(Natural Science)

基　　金：上海市教委科研创新项目(08YZ116);阜阳师范学院自然科学项目(2012FSKJ03)资助

摘　　要：采用平板扩散法对从健康大黄鱼肠道中分离筛选到的对病原弧菌有抑制作用的短小芽孢杆菌(Bacillus pumi-lus)X93胞外产物(Extracellular products,ECP)作用的环境影响因子进行了研究。结果显示,菌株X93胞外产物的抑菌活性强弱随着培养时间的不同而不同,胞外产物对pH条件有一定的耐受性,对热敏感,不耐高温,过高的NaCl能使其抑菌活性降低,蛋白酶K和不同浓度的FeCl3均对其抑菌活性产生影响,其胞外产物发挥最大抑菌作用的最适培养时间、温度、pH、盐度分别为48 h、28℃、pH 7和0.5%。The extracellular products （ECPs） of Bcuillus pumilus X93 were isolated from gastrointestinal tract of healthy cultured large yellow croaker （ Pseudosciaena crocea） , and the environmental factors on antibacterial activity of ECPs to two pathogenic vibfios were tested by flat diffusion method. The results show that the antibacterial activity varies with the culture time and ECPs not only have a certain tolerance to pH but also are sensitive to heat. Higher concentration of NaCl can reduce the antibacterial activity, and proteinase K and different concentrations of FeCl3 all have impacts on the antibacterial activity. The inhibition of the ECPs of strain X93 to two pathogenic vibrios is maximized when the optimal time, temperature, the pH, and salinity, are 48 h, 28 ℃, pH 7 and 0.5% respectively.

关 键 词：短小芽孢杆菌 胞外产物 抑菌活性 

分 类 号：S917.1[农业科学—水产科学]