尾状核区注射法建立Wistar大鼠C6胶质瘤模型的实验研究  

Establishment of a Wistar rat model bearing brain glioma C6 cells

作　　者：王青松[1] 林婷婷[1] 李安民[2] 曹朋[1] 史克珊[1] 李钢[2] 

机构地区：[1]海口市人民医院中南大学湘雅医学院附属海口医院神经外科,海南海口570208 [2]中国人民解放军总医院海南分院神经外科,海口三亚572013

出　　处：《海南医学》2013年第8期1093-1095,共3页Hainan Medical Journal

基　　金：国家自然科学基金(编号:39270668;30772245);海南省自然科学基金(编号:309108)

摘　　要：目的探讨尾状核区注射法建立Wistar大鼠C6胶质瘤模型的技术要点及成瘤效果。方法立体定向辅助下将C6细胞接种于Wistar大鼠右侧尾状核区,建立C6细胞胶质瘤鼠模型。共建立15只模型鼠,分别于接种的第7、14、21天行头颅MRI检查,3周后分批灌杀动物行病理HE切片观察、S100及GFAP免疫组织化学检测,了解肿瘤的生长特性和病理特征。对照组5只Wistar大鼠接种同体积培养液,同样方法进行检测。结果用本法建立Wistar大鼠C6胶质瘤模型15只,成功率为100%。磁共振扫描显示接种第2周时10只大鼠有肿瘤生长,MR表现为T2高信号;第3周时全部有肿瘤生长,瘤周水肿明显。病理、免疫组织化学显示大鼠C6胶质瘤生长特性和病理特征与人脑胶质瘤相似。结论该方法建立的胶质瘤模型稳定是进行胶质瘤研究的较好模型。Objective To establish a rat model bearing brain glioma, and to investigate its experimental application. Methods C6 cells were implanted into the unilateral brain hemisphere of 15 Wistar rats. MRI examination was performed on day 7, 14, 21. Three weeks after the implantation, the rates were killed for analysis of growth behaviors of the brain tumor and pathological features by HE, S100, GFAP. Results Fifteen Wistar rat model bearing brain glioma C6 cells were established, with the success rate of 100%. By day 14, the tumor became visible in 10/15 of the rats in at least two sections. By day 21, 15/15 of the rats showed space-occupying effect in the brain, and the tumor occupied over 1/2 of the hemisphere in 12/15 of the rats, and some tumor cells had migrated into the contralateral hemisphere. Conclusion C6 glioma implantation model is easy, economical, practical and applicable for the glioma experimental research.

关 键 词：C6胶质瘤模型 WISTAR大鼠 磁共振 

分 类 号：R-332[医药卫生]