柴芩承气汤对急性坏死性胰腺炎小鼠血清IL-6、IL-10水平及胰腺病理改变的影响  

Effect of Chai Qin Cheng Qi Decoction on serum interleukin-6,interleukin-10levels and pancreas pathological changes in acute necrotizing pancreatitis mice

作　　者：程征宇[1] 王明华[1] 罗榕[1] 祝华[1] 杨潇[1] 杨凌[1] 

机构地区：[1]峨眉山市人民医院消化内科,四川峨眉山614200

出　　处：《西部医学》2013年第4期498-500,共3页Medical Journal of West China

摘　　要：目的探讨柴芩承气汤(Chai Qin Cheng Qi Decoction,CQCQD)对急性坏死性胰腺炎(acute necrotizingpancreatitis,ANP)小鼠白细胞介素-6(interleukin-6,IL-6)、白细胞介素-10(interleukin-10,IL-10)水平及胰腺病理改变的影响。方法将30只昆明小鼠随机分成对照组、ANP组及CQCQD组,每组10只。ANP模型采用8%L-精氨酸溶液腹腔注射,4.0mg·g-1·1h-1×2制作。CQCQD组予以CQCQD制剂0.1ml/10g灌胃,1次/2h,观察其第6h的血清IL-6、IL-10水平及胰腺病理改变。结果 ANP组血清IL-6水平(1589.63±377.28)pg/ml高于对照组(927.46±210.42)pg/ml,P<0.05,也高于CQCQD组(1107.73±351.62)pg/ml,P<0.05,对照组血清IL-6水平与CQCQD组相比较,无统计学差异(P>0.05)。ANP组血清IL-10水平(920.64±101.68)pg/ml与对照组(1083.77±142.85)pg/ml相比较无统计学差异(P>0.05),ANP组血清IL-10水平低于CQCQD组(1177.84±201.72)pg/ml,P<0.05,对照组血清IL-10水平与CQCQD组相比较,无统计学差异(P>0.05)。ANP组胰腺病理评分(6.20±1.65)高于对照组(1.00±1.11),P<0.01,也高于CQCQD组(4.50±0.54),P<0.05。对照组胰腺病理评分低于CQCQD组(P<0.01)。结论 CQCQD可降低ANP小鼠IL-6水平,升高血清IL-10水平,减轻ANP小鼠胰腺的损伤。Objective To investigate Chai Qin Cheng Qi decoction （CQCQD） on serum interleukin-6（IL-6）, interleukin-10（IL-10） levels and pancreas pathological changes in acute necrotizing pancreatitis（ANP） mice. Methods Thirty mice were randomized into control group ANP group and CQCQD group with 10 rats in each group. L-arginine was administered intraperitoneally twice at doses of 4g/kg at intervals of 1 hour to induce the ANP of rats. All the rats in the CQCQD group were administrated the CQCQD at doses of 0. 1 ml/10g at intervals of 2 hours. The levels of serum IL- 6, IL-10 at the first 6 hours and the pathological changes of pancreas were observed. Results The levels of IL-6 in the ANP group（1589.63±377.28pg/ml） was higher than that in the control group（927.46±210. 42 pg/ml ）and CQCQD group （1107.73±351.62 pg/ml） （both, P〈0. 05）. However, there was no significant difference between the control group and CQCQD group （P〉0. 05）. The serum IL-10 in ANP group （920.64±101.68 pg/ml）was lower than that in CQCQD group（1177. 84±201. 72 pg/ml,P〈0.05）, and there was no significant difference between the ANP group and control group（1083.77±142.85 pg/ml, P〉0.05）. No statistical significance was obtained between the CQCQD group and control group （P〉0.05）. The pathological score of pancreas in ANP group （6.20±1.65） was higher than that in the control group （1.00±1.11, P〈0. 01）, and CQCQD group （4.50±0.54, P〈0.05）. The pathological score of pancreas in control group was lower than that in CQCQD group （P〈0.01）. Conclusion The findings of the study indicate that CQCQD can reduce the ANP mice IL-6 levels, elevated serum levels of IL-10 levels, and reduce the damage of ANP mice pancreas.

关 键 词：柴芩承气汤 急性坏死性胰腺炎 白细胞介素-6 白细胞介素-10 

分 类 号：R576[医药卫生—消化系统]