甲状旁腺激素相关蛋白对去卵巢大鼠牙槽骨的影响  

Effects of parathyroid hormone-related protein on the alveolar bone of ovariectomized rats

作　　者：李冰[1] 陈宁[1] 刘杰[1] 栾明亮[1] 

机构地区：[1]南京医科大学口腔医学研究所.附属口腔医院口腔颌面外科,江苏南京210029

出　　处：《口腔生物医学》2013年第1期23-26,共4页Oral Biomedicine

基　　金：江苏高校优势学科建设工程资助项目

摘　　要：目的:观察甲状旁腺激素相关蛋白(parathyroid hormone-related protein,PTHrP)对去卵巢大鼠牙槽骨的影响。方法:选用80只5月龄健康雌性大鼠,随机选其中60只行双侧卵巢摘除术(ovariectomized,OVX),另外20只行假手术不去卵巢;6周后,60只去除卵巢的大鼠再随机分为3组:PTHrP组注射PTHrP,雌激素组(E2组)注射苯甲酸雌二醇,安慰剂组(OVX组)注射生理盐水,每组20只;假手术的大鼠20只作为空白对照组(sham-OVX组)亦注射生理盐水。给药60d后,处死大鼠解剖分离右侧下颌骨,测定并比较4组大鼠磨牙区段牙槽骨骨密度(bone mineral density,BMD);颌骨第一磨牙区段制取切片,进行HE染色观察各组牙槽骨组织形态;切片进行免疫组化染色比较各组根周牙槽骨中Runt相关转录因子2(Runt-related transcription factor2,RUNX2)的表达。结果:BMD测定结果中,PTHrP组明显高于OVX组(P<0.05),且与E2组及sham-OVX组间无明显差异(P>0.05);HE染色显示,PTHrP组、E2组和sham-OVX组牙槽骨形态均较完整,而OVX组牙槽骨吸收较多;免疫组化染色显示,PTHrP组牙槽骨的RUNX2的表达明显高于于其余三组,差异有统计学意义(P<0.05)。结论:PTHrP可能对去卵巢大鼠牙槽骨的骨形成有促进作用。Objective：To investigate the effect of parathyroid hormone-related protein （PTHrP）on the alveolar bone of ovariectomized rats. Methods：80 female 5-month-old rats were selected. Among them 60 rats were ovariectomized （OVX） and the other 20 rats were sham-ovariectomized （sham-OVX）. Six weeks later the OVX rats were further divided into three groups randomly, each grouop consisting of 20 rats： the PTHrP treated group （PTHrP group） which was injected with PTHrP, the estrogen treated group （E2 group） which was injected with Estradiol benzoate, and the placebo group （OVX group）which was with injected saline,while the 20 sham-OVX rats （sham-OVX group） were also injected with saline as the blank control group. 60 days later, all the rats were sacrificed and their fight mandibles were obtained for measuring bone mineral density （BMD） of molars region. Besides ,the alveolar bones of the first mo- lars were cut into tissue spices for histomorphological observation and immunohistoehemieal staining of Runt-related transcription factor 2 （RUNX2）. Results：The BMD measure indicated that the PTHrP group had a higher BMD value significantly than the OVX group （P 〈 0.05 ） , with no less than the E2 group or even the sham-OVX group （ P 〉 0.05 ）. The histomorphologieal observation showed the morphology of alveolar bone of the PTHrP group approximated the E2 group and the sham-OVX group, without bone loss as apparent as the OVX group. Moreover,the analysis of immunohistochemieal staining suggested that the RUNX2 expression in PTHrP group was more than either of the other groups, which showed significant statistical differences （ P 〈 0.05 ）. Conclusions ： PTHrP may have an anabolic effect on the alveolar bone of the ovariectomized rats.

关 键 词：甲状旁腺激素相关蛋白 去卵巢大鼠 牙槽骨 

分 类 号：R780.2[医药卫生—口腔医学]