MTAP对人乳腺癌细胞侵袭和迁移的影响  

Effects of knockdown of MTAP on invasion and migration in human breast cancer cells

作　　者：姜雨刚[1] 张安秦[1] 朱彩霞[1] 卢奕嘉[1] 王颀[1] 

机构地区：[1]广东省妇幼保健院乳腺疾病防治中心,广州511400

出　　处：《国际外科学杂志》2013年第3期160-164,共5页International Journal of Surgery

摘　　要：目的观察甲硫腺苷磷酸化酶（MTAP）对人乳腺癌细胞侵袭和迁移能力的影响。方法利用RNA干扰技术下调人乳腺癌细胞系MCF-7细胞中MTAP的表达，CCK-8试剂盒检测细胞增生能力，Tr-answell检测MCF-7细胞的侵袭及迁移能力。Westernblotting检测MCF-7细胞中MTAP及基质金属蛋白酶1（MMP1）的表达。实验分组：空白对照组、阴性对照组、MTAP—siRNA实验组。结果RNA干扰技术下调MCF-7细胞中MTAP表达后，促进了MCF-7细胞的增生，CCK-8检测显示，MATP—siRNA实验组450nm吸光度在24h、48h、72h分别是空白对照组的（112．3±11．9）％、（144．4±8．4）％、（169．3±9．4）％。Tran—swell侵袭实验结果显示，空白对照组、阴性对照组及MTAP—siRNA实验组在570nm吸光度值分别为0．49±0．06、0．45±0．07、0．87±0．07。细胞迁移实验显示空白对照组、阴性对照组及MTAP—siRNA实验组在570nm吸光度值分别为0．46±0．06、0．49±0．08、0．75±0．07。在下凋MCF-7细胞中MTAP的表达后，West—erRblotting检测发现MMP1的表达上调。结论MTAP表达下调可以增强人乳腺癌细胞MCF-7的侵袭及迁移能力，并可能与MMP1的表达上调有关。± Objective To investigate the effects of methylthioadenosine phosphorylase （MTAP） on invasion and migration in breast cancer cells. Methods Human breast cancer cell line MCF-7 cells were treated with MTAP-targeted siRNA to diminish MTAP mRNA. MCF-7 cells proliferation was evaluated by cell counting kit-8, the analysis of ceils invasion and migration was performed using Transwell chamber. The expressions of MTAP and matrix metalloproteinase 1 （MMP1） in cell extracts were detected by Western blotting. The experimental divided into blank contrd group, negative control group, MTAP-siRNA experimental group. Results The MCF-7 cells growth was promoted after knockdown the MTAP mRNA. MATP-siRNA experimental group 450 nm absorbance values at 24 h, 48 h and 72 h of the control group were （ 112.3 ± 11.9） %, （ 144.4 ± 8.4） %, （ 169.3 ± 9.4） % respectively. Cell invasion analysis by Transwell chamber showed 570 nm absorbance values were 0.49 -± 0.06（ control）, 0.45 ± 0.07 （ negative control） and 0.87 ± 0.07 （ MTAP-siRNA） respectively. Cell migration analysis by Transwell chamber showed 570 nm absorbance values were 0.46 ± 0.06（ control）, 0.49 ± 0.08（ negative control） and 0.75 ± 0.07（MTAP-siRNA） respectively. The expression of MMP1 in MCF-7 cells was upregulated after knockdown the MTAP mRNA. Conclusion The knockdown of MTAP in MCF-7 cell can increase the cells invasion and migration, and this may involve the the MMP1.

关 键 词：乳腺肿瘤 癌 甲硫腺苷磷酸化酶 基质金属蛋白酶1 增生 侵袭 迁移 

分 类 号：R737.9[医药卫生—肿瘤]