FISH检测宫颈细胞hTERC基因在宫颈癌筛查中的价值  

Evaluation of Testing Hterc Gene with FISH in Screening of Cervical Cancer

作　　者：米贤军[1] 吴秋良[2] 钟守军[1] 段立锋[1] 刘晓萍[3] 徐秀梅[1] 陈昂[1] 

机构地区：[1]南方医科大学附属中山市博爱医院病理科,广东省中山市528400 [2]中山大学附属肿瘤医院病理科 [3]中南大学湘雅医学院肿瘤研究所

出　　处：《中华全科医学》2013年第4期504-505,541,共3页Chinese Journal of General Practice

基　　金：卫生部科研基金资助项目(WKJ2007-3-001)

摘　　要：目的探讨FISH检测宫颈细胞hTERC基因表达及在宫颈癌和癌前病变筛查中的价值。方法收集300例宫颈细胞标本,经病理活检确诊:正常或炎症30例、CIN1 100例、CIN2 60例、CIN3 60例、SCC 50例。用FISH检测宫颈细胞hTERC基因扩增情况,以正常标本的检测结果建立阈值。结果正常阈值为5.91%,在不同病理学分组中扩增阳性率分别为:CIN1 17.00%(17/100)、CIN2 66.67%(40/60)、CIN3 88.33%(53/60)、SCC 100.00%(50/50)。各组hTERC基因阳性率与正常对照组相比差异均有统计学意义(P<0.01),随着病理级别的上升,其阳性率不断升高。CIN2、CIN3和SCC的阳性率显著高于正常及CIN1者(P<0.01)。结论在不同级别CIN中有hTERC基因扩增,随着病理级别上升,阳性率也逐步升高。FISH检测hTERC基因可以作为宫颈癌和癌前病变早期筛查的手段,预测CIN进展、指导治疗及评估预后的重要指标。Objective To investigate the expression of human telomerase RNA component（hTERC） gene in cervical cells and its clinical application in the screening of cervical cancer.Methods Total 300 samples of cervical cells were collected,then the results of pathological biopsy confirmed that 30 cases were normal or with inflammation,100 cases with grade 1 cervical intraepithelial neoplasia（CIN1）,60 cases with CIN2,60 cases with CIN3 and 50 cases with cervical squamous cell carcinoma were included.Fluorescence in situ hybridization（FISH） was used to detect the amplification of hTERC gene in cervical cells,the detection results of normal samples were used to establish threshold.Results The normal threshold was 5.91%,the positive rates of hTERC gene amplification in patients with CIN1,CIN2,CIN3 and cervical squamous cell carcinoma were 17.00%（17/100）,66.67%（40/60）,88.33%（53/60） and 100.00%（50/50）,respectively.Compared with control group,the positive expression rates of hTERC gene in every group was significantly different（P0.01）.The positive rate of hTERC gene amplification increased gradually with the increase of degree of cervical lesions.The positive rates of hTERC gene amplification in patients with CIN2,CIN3 and SCC were significantly higher than those in normal cases and CIN1（P0.01）.Conclusion hTERC gene amplification may occurred in different cervical lesions,the positive rate of hTERC gene increases gradually with the increase of degree of cervical lesions,which can be used as not only early screening methods of cervical cancer and precancer disease but also an important indicator to predict the development of CIN,direct therapy and prognosis evaluation.

关 键 词：荧光原位杂交技术 HTERC基因 宫颈癌 宫颈上皮内瘤变 

分 类 号：R737.33[医药卫生—肿瘤] R730.43[医药卫生—临床医学]