表没食子酸儿茶素没食子酸酯(EGCG)在小鼠肾脏分布研究  

Study on the distribution of epigallocatechin gallate (EGCG) in mouse’s kidney

作　　者：刘洋[1] 莫丽[1] 张新亭[1] 周宁宁[1] 王梦馨[1] 韩宝瑜 葛建[1,2] 

机构地区：[1]中国计量学院生命科学学院,浙江杭州310018 [2]浙江省生物计量及检验检疫技术重点实验室,浙江杭州310018

出　　处：《中医临床研究》2013年第4期1-2,共2页Clinical Journal Of Chinese Medicine

基　　金：国家自然科学基金资助(31100499);2012年浙江省大学生科研创新团队资助项目(新苗人才项目);中国计量学院第十六届学生科研计划项目

摘　　要：本文应用RP-HPLC建立了小鼠肾脏中儿茶素EGCG检测方法,同时探索儿茶素EGCG在小鼠肾脏组织分布特征。小鼠腹腔注射后,不同时间点采集肾脏组织,RP-HPLC检测其中EGCG含量,计算儿茶素EGCG在肾脏中的分布。研究结果表明所建立的HPLC检测方法在0.5～200mg/L范围内线性关系良好(r>0.99),平均回收率85.0%以上,日内和日间精密度均低于10.0%。该方法操作简便、灵敏、准确,适合于肾脏组织分布研究。The study was designed to investigate the distribution of epigallocatechin gallate （EGCG） in mouse kidney, based on establishing a RP-HPLC method for determination of EGCG Kidney tissue was collected after administeration by intraperitoneal injection. Then EGCG concentrations were calculated by RP-HPLC method mentioned above. The results showed that there was a good linearity over the range 0.5-200.0 mg/L （r〉0.99）. Then the recoveries were more than 85.0% in kidney samples, and the intra- and inter-day coefficients of variation were less than 10.0% in all cases. The RP-HPLC method was specific, simple, sensitive and accurate. And this method was successfully applied to EGCG distribution in mouse kidney.

关 键 词：儿茶素EGCG 肾脏分布 高效液相色谱 

分 类 号：R222.19[医药卫生—中医基础理论]