非小细胞肺癌组织中PTEN基因与Ki67的表达及意义  

Expression and Significance of PTEN Gene and Ki67 in Non-small Cell Lung Cancer

作　　者：张梓威[1] 于海燕[1] 窦佳鸣[1] 孙杨帆[1] 纪勇[1] 

机构地区：[1]南京医科大学附属无锡市精神卫生中心,江苏无锡214400

出　　处：《现代生物医学进展》2013年第5期942-945,共4页Progress in Modern Biomedicine

摘　　要：目的:探讨第10染色体同源丢失性磷酸酶-张力蛋白酶基因(PTEN)、Ki67在非小细胞肺癌组织中的表达及其相关性。方法:用免疫组化方法检测67例非小细胞肺癌组织以及41例癌旁正常肺组织中PTEN基因、Ki67蛋白的表达,并分析其与各临床病理指标及细胞增殖之间的相关性。结果:PTEN基因在67.16%(45/67)的非小细胞肺癌中阳性表达率为32.84%(22/67),而在正常肺组织中阳性表达率为82.97%,显著高于非小细胞癌组织。PTEN基因的表达与组织类型、细胞分化程度、淋巴结转移密切相关(分别为X2=5.44,P=0.019;X2=4.740,P=0.029;X2=4.5l,P=0.034),在鳞癌和低分化癌中PTEN基因失表达率或表达减少率较高。肺癌中Ki67的过度表达与肺癌的临床分期、淋巴结转移相关(X2=6.90,P=0.009;X2=5.68,P=0.017),PTEN蛋白阳性表达与Ki67负相关(r=-0.239,P<0.05)。细胞增殖指数越高,PTEN基因表达越少,二者呈负相关(r=-0.252,P<0.05)。结论:PTEN蛋白表达的缺失与非小细胞肺癌的恶性侵袭有关。联合检测PTEN与Ki67的表达可有助于判断非小细胞肺癌的预后。Objective： To study the expression of gene PTEN,Ki67 Protein in non-small cell lung cancer（NSCLC） and the relationships with cell proliferation.Methods： The protein expressions of PTEN and Ki67 were evaluated by immunohistochemistry in 67 NSCLC patients and 41 adjacent normal lung tissue.The correlations with histopathologic and cell proliferation were also analyzed.Results： The positive expression of PTEN was in 32.84% of NSCLC,while it was normal in 82.93% of 41 adjacent normal lung tissues.The expression of PTEN was strongly correlated with tumor histology,histologic grade and lymph node metastasis（X2=5.44,P=0.019;X2=4.740,P=0.029;X2=4.5l,P=0.034）,reduced PTEN expression was higher in squamous cell carcinomas and in poorly differentiated tumors.The overexpression of Ki67 was associated with TNM stage and lymph node metastasis（X2=6.90,P=0.009;X2=5.68,P=0.017）.PTEN expression was negatively related to the overexpression of Ki67（r=-0.239,P0.05）.PTEN expression intensity was negatively related to the high index of cell proliferation（r=-0.252,P0.05）.Conclusion： The loss of PTEN expression plays different roles in tumorigenesis of NSCLC and in related to high aggressiveness of tumors.The loss of PTEN expression and the overexpression of Ki67 can estimate the prognosis of NSCLC.

关 键 词：非小细胞肺癌 磷酸酶-张力蛋白基因 KI67 免疫组织化学 

分 类 号：R734.2[医药卫生—肿瘤]