SNAP-25在大鼠蛛网膜下腔出血后脑组织中的表达研究  

The Expression of SNAP-25 in Rat Brain Tissue for Subarachnoid Hemorrhage

作　　者：胡童[1] 王中[1] 陈罡[1] 李琦[1] 

机构地区：[1]苏州大学附属第一医院神经外科,江苏苏州215006

出　　处：《现代生物医学进展》2013年第4期624-626,共3页Progress in Modern Biomedicine

基　　金：国家自然科学基金项目(81100872)

摘　　要：目的:观察蛛网膜下腔出血(SAH)后突触小体相关蛋白(SNAP-25)在大鼠脑组织中的表达变化。方法:42只成年健康雄性SD大鼠,随机分为正常组、对照组、SAH后1、3、5、7、14天共7组,经大鼠自体股动脉(尾动脉)非肝素化动脉血注入视交叉池致蛛网膜下腔出血模型。蛛网膜下腔出血后1、3、5、7、14天后处死大鼠,取颞叶皮层、海马、小脑做标本,Western blot及免疫组化测定SNAP-25,采用SPSS16.0软件分析。结果:与正常组相比,对照组无明显差异(P>0.05);SAH组中SAH后1天的SNAP-25蛋白水平降低明显(P<0.05);SAH后3天蛋白水平达到最低值(P<0.01);SAH后5、7天的SNAP-2蛋白水平虽降低但呈逐渐上升趋势(P<0.05);SAH后14天的SNAP-2蛋白水平与正常组相比无明显差异(P>0.05)。结论:SNAP-2在SAH后72小时明显下降,后逐渐上升,至14天时基本恢复正常。Objective： To investigate the expression of SNAP-25 in brain tissue for subarachnoid hemorrhage （SAH）. Methods： 42 male Sprague Dawley rats were randomly divided into seven groups： normal group, control group, 1 days （D1）, 3 days （D3）, 5 days （D5）, 7 days （D7）, 14 days （D14） after SAH. All SAH animals were subjected to injection of 0.3 mL fresh arterial non-heparinized blood into prechiasmatic cistem. Brain samples including cerebral cortex, hippocampus and cerebellum were extracted in 1, 3, 5, 7, 14days after SAH. The expressions of SNAP-25 were detected by western blot and immunohistochemistry. Results： There was no statistically signifi- cant difference in SNAP-25 protein expression between normal group and control group （P〉0.05）; The level of SNAP-25 in 1 days after SAH reduced markedly, and reduced the lowest level in 3 days after SAH （P〈0.01）; But SNAP-25 level rose again in 5 and 7 days after SAH （P〈0.05）; When in 14 group after SAH, the SNAP-25 level reached the normal （P〉0.05）. Conclusion： SNAP-25 level decreased sig- nificantly in 3 days after SAH, but then rose gradually, and went up to normal in 14 days.

关 键 词：SNAP-25 蛛网膜下腔出血 WESTERN BLOT 免疫组化 SD大鼠 

分 类 号：Q95-3[生物学—动物学] R743.35[医药卫生—神经病学与精神病学]