氯喹对戊四氮慢性致痫大鼠脑皮质及海马腺苷激酶表达的影响  

Effects of chloroquine on adenosine kinase expressions in cerebral cortex and hippocampus of rat brains with epilepsy induced by pentylenetetrazole

作　　者：于宁[1] 朱玉红[1] 荆丽丽[3] 杨东霞[2] 吴淑华[2] 

机构地区：[1]山东省滨州医学院附属医院病理科,滨州256603 [2]山东省滨州医学院病理教研室,滨州256603 [3]山东省滨州医学院附属烟台牟平医院病理科,烟台264100

出　　处：《中华神经医学杂志》2013年第4期343-346,共4页Chinese Journal of Neuromedicine

摘　　要：目的观察氯喹对戊四氮致痫大鼠皮质和海马区腺苷激酶（AOK）表达的影响，探讨ADK与癫痫发作的关系及氯喹在癫痫发生过程中的作用。方法30只健康雄性SD大鼠按照随机数字表法分为对照组、戊四氮fPTZ）致痫组和氯喹干预组，每组10只。观察大鼠行为学表现，记录其脑电改变，采用免疫组化法检测3组大鼠皮质和海马区ADK的表达。结果对照组大鼠无癫痫发作，PTZ致痫组大鼠出现Racine评分中Ⅳ～V级严重发作．氯喹干预组大鼠出现I～Ⅲ级发作．差异有统计学意义（P〈0．05）。PTZ致痫组大鼠脑电记录呈频发高幅的痫样波，氯喹干预组大鼠脑电记录显示慢波、小棘波。PTZ致痫组大鼠脑内ADK表达明显增强，以海马区最为显著，与对照组相比差异均有统计学意义（P〈0．05）。氯喹干预组大鼠脑内ADK表达降低，但距离正常水平仍有较大差距，与对照组相比差异有统计学意义（P〈0．05）。结论癫痫脑组织中存在ADK的表达异常．氯喹可以抑制这种表达．有效控制癫痫发作。Objective To observe the effects of chloroquine on adenosine kinase （ADK） expressions in the cerebral cortex and hippocampus of rats with epilepsy induced by pentylenetetrazole, explore the relationship between ADK and seizures and discuss the role of chloroquine in epileptogenesis. Methods Thirty healthy male adult Sprague-Dawley （SD） rats were randomly divided into control group （n=10）, pentylenetetrazole （PTZ） inducement group （n=10） and chloroquine intervention group （n=10）. The behavior and electroencephalogram changes of the rats were observed and recorded. The expressions of ADK in the cerebral cortex and hippocampus were examined with immunohistochemistry. Results The rats in the control group showed no seizure activity. Severe seizure activity was observed in PTZ inducement group 0V-V degree）, while slight seizure activity （I-III degree） appeared in the rats of chloroquine intervening group （P〈0.05）. Electroencephalogram studies showed high amplitudes with fast frequencies in the PTZ imducement group, while low amplitudes and slow frequencies in the chloroquine intervention group. The expressions of ADK in the PTZ inducement group increased, especially in the hippocampus area, as compared with those of the control group （P〈0.05）; the expressions of ADK in the chloroquine intervention group were reduced as compared with the control group, with significant differences （/〈0.05）. Conclusion In rat brains with seizures, the expressions of ADK are abnormal; and chloroquine, by inhibiting the release of ADK, can alleviate the seizure activities induced by PTZ in rats.

关 键 词：腺苷激酶 氯喹 星形胶质细胞 戊四氮 癫痫 

分 类 号：R742.1[医药卫生—神经病学与精神病学]