ROS融合基因不是中国胆管癌病人的常见表达基因(英文)  

ROS kinase fusions are not common in Chinese patients withcholangiocarcinoma

作　　者：刘鹏敏[1] 武雅君[1] 孙丽[1] 左强[1] 石敏[1] 

机构地区：[1]南方医科大学南方医院肿瘤科,广东广州510515

出　　处：《南方医科大学学报》2013年第4期474-478,共5页Journal of Southern Medical University

基　　金：Supported by Wu Jieping Medical Foundation(Grant number 3206740.10017)~~

摘　　要：目的探讨胆管癌中ROS融合基因的表达情况。方法收集经甲醛固定和石蜡包埋的I-IV期胆管癌组织,采用RT-PCR对胆管癌组织进行ROS融合基因(FIG-ROS、SLC34A2-ROS、CD74-ROS)检测。SERPINA1作为参照基因。结果在56个中国人胆管癌样本中,80.4%(45/56)表达SERPINA1,在这些样本中没有任何一种ROS融合基因表达。结论 ROS融合基因在中国胆管癌病人中不是常见表达基因。Objective To investigate the expressions of different forms of ROS fusions in Chinese patients withcholangiocarcinoma（CCA）.Methods RT-PCR was employed to examine formalin-fixed and paraffin-embedded CCA samplesfrom stage I-IV patients for detection of ROS fusions involving Fused in Glioblastoma（FIG）,solute carrier protein（SLC34A2）and major histocompatibility complex class II invariant chain（CD74）.Serpin peptidase inhibitor clade A member 1（SERPINA1） was detected as the reference gene.Results In all the 56 CCA samples,80.4%（45/56） were positive for SERPINA1expression as evaluable samples.Of these evaluable samples,none expressed the ROS fusions.Conclusion ROS fusions are notcommon in Chinese CCA patients.

关 键 词：ROS融合基因 胆管癌 RT-PCR 

分 类 号：R735.8[医药卫生—肿瘤]