HSP90β在小鼠生殖系统中的表达及调控  

Expression of heat shock protein 90β and its regulation in the reproductive system of male mice

作　　者：戎成婷[1] 韩俊岭[2] 杜振宁[1] 

机构地区：[1]烟台大学药学院,山东烟台264005 [2]宁夏医科大学研究生院,宁夏银川750004

出　　处：《南方医科大学学报》2013年第4期491-495,共5页Journal of Southern Medical University

基　　金：国家自然科学基金(31071262)~~

摘　　要：目的研究热休克蛋白90β(HSP90β)在小鼠睾丸、附睾、精子中的表达及调控机制。方法从mRNA和蛋白水平上对HSP90β在小鼠睾丸和附睾组织细胞中及精子上表达及定位进行分析,同时对其基因在雄性生殖中的表达调控进行初步探索。结果 HSP90βmRNA在小鼠附睾中的表达量比在睾丸中高。在小鼠精子中定位于顶体部位。其基因在去势小鼠的附睾中表达降低,补充睾酮后又恢复正常,在发育中小鼠的睾丸、附睾中表达也有变化。结论 HSP90β在小鼠精子形成及受精中可能发挥重要作用;其在去势小鼠附睾中和在出生后不同阶段小鼠睾丸、附睾中的表达模式,说明其基因表达受激素调控和发育调控的影响。Objective To study the expression and regulation of heat shock protein 90β（HSP90β） in the testis,epididymis and sperms of mice.Methods The localization and expression of HSP90β mRNA and protein were investigated in the testis, epididymis and sperms of mice,and the regulation of HSP90β in the male reproductive system was explored.Results HSP90β was expressed at a higher level in the epididymis than in the testis.In the sperms of the mice,HSP90β was localized in the acrosome area.The expression of HSP90β in mouse epididymis decreased after castration and recovered the normal level after testosterone treatment.HSP90β expression in the testis and epididymis also underwent changes during the postnatal development of the mice.Conclusion HSP90β may play an important role in spermiogenesis and fertilization,and its expression pattern in the epididymis after castration and during the postnatal development suggests its regulation by hormones and development.

关 键 词：热休克蛋白90β 睾丸 附睾 精子 调控 

分 类 号：R363[医药卫生—病理学]