牛血清白蛋白乳酸-羟乙酸共聚物缓释微球制备工艺的优化  

Optimization of preparation of bull serum albumin and poly lactide-co-glycolide acid controlled-release microparticles

作　　者：柴雪[1] 屈铁军[1] 李伟[1] 王蕾[1] 徐波[1] 张亚庆[1] 

机构地区：[1]第四军医大学口腔医学院,陕西西安710032

出　　处：《牙体牙髓牙周病学杂志》2013年第4期270-274,共5页Chinese Journal of Conservative Dentistry

基　　金：国家自然科学基金资助项目(31200738);陕西省社发攻关项目(2011K14-03-07)

摘　　要：目的:以牛血清白蛋白(Bull serum albumin,BSA)为模型蛋白,聚乳酸-羟乙酸共聚物(Poly1actic-co-glycolic acid,PLGA)为载体,探索并优化微球的制备工艺。方法:采用复乳溶剂挥发法制备BSA-PLGA微球,激光粒度分析仪测量微球粒径,以微量BCA法测定微球的蛋白含量并计算包封率,进行体外释放,测定微球的24 h累积释放量。探索BSA投药量、外水相PVA(Polyvinyl alcohol)浓度、NaCl浓度及复乳匀质速度对微球包封率、24 h释放量和粒径的影响。结果:通过正交试验,在减少BSA-PLGA微球突释的情况下,兼顾微球粒径大小,通过适当增加BSA的投药量提高BSA-PLGA微球的包封率,得到微球的优化制备条件为BSA投药量10 mg,外水相PVA浓度10 g/L,NaCl浓度50 g/L,复乳的匀质速度10 000 r/min。结论:通过控制不同制备因素,可得到包封率较高、24 h突释量较小,粒径适宜的BSA-PLGA微球。AIM： To optimize the method of preparation of microspheres using bull serum albumin （BSA） as the model protein and poly lactic-co-glycolic acid （PLGA） as the controlled-release carrier. METHODS： The BSA-PLGA microspheres were prepared using a （water-in-oil）-in-water emulsion solvent evaporation technique. Mi- crosphere particle size was measured by laser particle size analyzer. Trace BCA method was used for determination of the protein content in the microspheres. Encapsulating rate was calculated. RESULTS： After various orthogonal tests, it was found that the optimal microsphere preparation condition was with 10 mg BSA, 1% Polyvinyl alcohol （PVA） , 5%NaC1 and at a stirring rate of 10 000 r/min for secondary emulsion . ONCLUSION： The optimized preparation method may produce BSA-PLGA microspheres with increased encapsulation efficiency, reduced 24 h burst release and appropriate particles size.

关 键 词：聚乳酸-羟乙酸共聚物 牛血清白蛋白 正交试验 微球 

分 类 号：R780.2[医药卫生—口腔医学]