Zeolite-based hemostat QuikCIot releases calcium into blood and promotes blood coagulation in vitro  

作　　者：Jing LI Wei CAO Xiao-xing LV Li JIANG Yue-jun LI Wang-zhou LI Shao-zong CHEN Xue-yong LI 

机构地区：[1]Department of Burns and Plastic Surgery, Tangdu Hospital, the Fourth Military Medical University, Xi-an 710038, China [2]Depart- ment of Interventional Radiology, Tangdu Hospital, the Fourth Military Medical University, Xi-an 710038, China

出　　处：《Acta Pharmacologica Sinica》2013年第3期367-372,共6页中国药理学报（英文版）

摘　　要：Aim： To examine the changes in electrolyte concentrations after addition of zeolite-based hemostat QuikCIot in blood and the effects of zeolite on blood coagulation in vitro. Methods： Fresh blood was taken from healthy adult volunteers and sheep, and the electrolyte concentrations in blood were measured using a blood electrolyte analyzer. Zeolite Saline Solution （ZSS） was prepared by addition of 2 g zeolite to 0.9% NaCl solution （4, 8, or 16 mL）. The electrolytes in ZSS were measured using inductively coupled plasma atomic emission spectroscopy. The prothrombin time （PT） and activated partial thromboplastin time （APTI＇） of blood were measured using the test tube method. The activated clotting time （ACT） and clotting rate （CR） of blood were measured with Sonoclot Coagulation and Platelet Function Analyzer. Results： Addition of zeolite （50 and 100 rag） in 2 mL human blood significantly increased Ca2＋ concentration, while Na^＋ and K＋ concentrations were significantly decreased. Addition of zeolite （50 and 100 mg） in 0.9% NaCl solution （2 mL） caused similar changes in Ca2＋ and Na^＋ concentrations. Si4~ （0.2434 g/L） and AI3＋ （0.2575 g/L） were detected in ZSS （2 g/8 mL）, Addition of ZSS in sheep blood shortened APTT in a concentration dependent manner, without changing PT. ZSS or aqueous solution of CaCl2 that contained Ca2＋ concentration identical to that of ZSS significantly shortened ACT in human blood without significantly changing CR, and the effect of ZSS on ACT was not significantly different from that of CaCl2. Conclusion： Zeolite releases Ca2＋ into blood, thus accelerating the intrinsic pathway of blood coagulation and shortening the clot formation time.Aim： To examine the changes in electrolyte concentrations after addition of zeolite-based hemostat QuikCIot in blood and the effects of zeolite on blood coagulation in vitro. Methods： Fresh blood was taken from healthy adult volunteers and sheep, and the electrolyte concentrations in blood were measured using a blood electrolyte analyzer. Zeolite Saline Solution （ZSS） was prepared by addition of 2 g zeolite to 0.9% NaCl solution （4, 8, or 16 mL）. The electrolytes in ZSS were measured using inductively coupled plasma atomic emission spectroscopy. The prothrombin time （PT） and activated partial thromboplastin time （APTI＇） of blood were measured using the test tube method. The activated clotting time （ACT） and clotting rate （CR） of blood were measured with Sonoclot Coagulation and Platelet Function Analyzer. Results： Addition of zeolite （50 and 100 rag） in 2 mL human blood significantly increased Ca2＋ concentration, while Na^＋ and K＋ concentrations were significantly decreased. Addition of zeolite （50 and 100 mg） in 0.9% NaCl solution （2 mL） caused similar changes in Ca2＋ and Na^＋ concentrations. Si4~ （0.2434 g/L） and AI3＋ （0.2575 g/L） were detected in ZSS （2 g/8 mL）, Addition of ZSS in sheep blood shortened APTT in a concentration dependent manner, without changing PT. ZSS or aqueous solution of CaCl2 that contained Ca2＋ concentration identical to that of ZSS significantly shortened ACT in human blood without significantly changing CR, and the effect of ZSS on ACT was not significantly different from that of CaCl2. Conclusion： Zeolite releases Ca2＋ into blood, thus accelerating the intrinsic pathway of blood coagulation and shortening the clot formation time.

关 键 词：HEMOSTATICS QuikClot zeolites electrolytes CALCIUM blood coagulation prothrombin time activated partial thromboplastintime activated clotting time clotting rate 

分 类 号：TQ124.41[化学工程—无机化工] Q98[生物学—人类学]