Separation of four-lol drugs by HPLC with new bonded chiral stationary column  

作　　者：ZHOU Jie PEI WenJuan SU Nan LIU Qian FU GuangJun ZHANG ZhenZhong 

机构地区：[1]School of Pharmacy, Zhengzhou University [2]Research Center of Nanotechnology for Drug, Zhengzhou University

出　　处：《Chinese Science Bulletin》2013年第11期1267-1273,共7页

摘　　要：Separation of betaxolol, bevantolol, metoprolol, bisoprolol with new bonded chiral stationary (3,5-dimethylcabamate cellulose) Chiralpak IB column was investigated. The factors, such as mobile phase composition and the ratio, column temperature, flow rate and the proportion of chiral additives, were also investigated. The optimal mobile phase compositions of betaxolol, bevantolol, metoprolol, bisoprolol were n-hexane/ethanol (95/5, v/v; 0.2% DEA); n-hexane/ethanol (90/10, v/v; 0.1% DEA); hexane/ethanol (60/40, v/v; 0.1% DEA); n-hexane/ethanol (95/5, v/v; 0.2% DEA), respectively. The optimal temperature was 30℃ with a flow rate of 0.8 mL min-1 . The detection wavelength, by 1200VWD UV detector, of betaxolol, bevantolol, metoprolol, bisoprolol was 259, 274, 224 and 223 nm, respectively. Experimental results demonstrated that baseline separation (Rs>1.5) of the betaxolol, bevantolol, metoprolol, bisoprolol enantiomers were obtained with new bonded Chiralpak IB column. This method can be used for analysis and detection of these four drugs.Separation of betaxolol, bevantolol, metoprolol, bisoprolol with new bonded chiral stationary （3,5-dimethylcabamate cellulose） Chiralpak IB column was investigated. The factors, such as mobile phase composition and the ratio, column temperature, flow rate and the proportion of chiral additives, were also investigated. The optimal mobile phase compositions of betaxolol, bevantolol, metoprolol, bisoprolol were n-hexane/ethanol （95/5, v/v; 0.2% DEA）; n-hexane/ethanol （90/10, v/v; 0.1% DEA）; hexane/ethanol （60/40, v/v; 0.1% DEA）; n-hexane/ethanol （95/5, v/v; 0.2% DEA）, respectively. The optimal temperature was 30~C with a flow rate of 0.8 mL min-1. The detection wavelength, by 1200VWD UV detector, of betaxolol, bevantolol, metoprolol, bisoprolol was 259, 274, 224 and 223 nm, respectively. Experimental results demonstrated that baseline separation （Rs〉l.5） of the betaxolol, bevantolol, metoprolol, bisoprolol enantiomers were obtained with new bonded Chiralpak IB column. This method can be used for analysis and detection of these four drugs.

关 键 词：液相色谱分离 手性添加剂 药物 美托洛尔 比索洛尔 检测波长 UV检测器 DEA 

分 类 号：R914[医药卫生—药物化学]