基于光子悬浮列阵多元检测肿瘤标志物的初步研究  

Preliminary of study multiple detection of tumor markers based on photonic suspension array

作　　者：卢颖辉[1] 何杰 田小强[1] 常立功[1] 黄培林[1] 

机构地区：[1]东南大学医学院,江苏南京210009 [2]安徽医科大学附属省立医院病理科,安徽合肥230000

出　　处：《东南大学学报（医学版）》2013年第2期150-153,共4页Journal of Southeast University(Medical Science Edition)

基　　金：东南大学生物电子国家重点实验室开放项目;国家自然科学基金资助项目(30973475)

摘　　要：目的:探讨基于二氧化硅胶体晶体微球(SCCBs)微列阵多元检测技术在多元检测肿瘤标志物中的价值。方法:以SCCBs为载体,应用免疫双抗夹心法同时检测人胶质瘤细胞蛋白提取物中Bcl-2、p21蛋白的水平,并与传统ELISA方法相比较。结果:用基于SCCBs微列阵多元检测技术同时检测并用3倍信噪比计算Bcl-2、p21蛋白的线性范围分别是9～180 ng.ml-1和0.05～1 ng.ml-1,最低检测限分别是1 ng和0.049ng。样品检测结果与ELISA方法检测结果无明显差异,提示此方法检测微量蛋白较传统方法具有高通量、高灵敏度等特点。结论:基于SCCBs的多元检测方法具有潜在的临床应用价值。Objective： To study the multi-detection technology of tumor markers based the silica colloidal crystal beads（SCCBs）,and its value in the multi-detection technology of tumor markers.Methods： Using SCCBs as a carrier and immuno sandwich method, Bcl-2 protein and p21 protein extracted from human glioma cells were detected and compared with traditional ELISA method.Results： Simultaneously detected Bcl-2 protein and p21 protein based on the SCCBs microarray multiplex detection technology were within a linear range of 9-180 ng·ml-1,0.05-1 ng·ml-1,respectively, with a minimum detectable level of 1 ng and 0.049 ng at 3 σ,respectively.The proposed array showed that the storage stability and the accuracy for sample detection were acceptable.The new method,compared to traditional methods,had high-throughput,high- sensitivity characteristics. Conclusion： The multiple detection method based SCCBs has a potential value of clinical application.

关 键 词：光子悬浮列阵 多元检测 二氧化硅胶体晶体微球 BCL-2蛋白 P21蛋白 

分 类 号：R730.43[医药卫生—肿瘤]