^(252)锎中子射线对宫颈癌组织中VEGF和Gli-1表达的影响  

Influence of californium-252 neutron ray on the expressions of the VEGF and Gli-1 in tumor tissues from cervical cancer patients

作　　者：刘兴京[1] 钱莘[1] 李锋[1] 陈娟[1] 宋妮[1] 侯兵[1] 黄作平[1] 宋晓军[1] 

机构地区：[1]武警广东省总队医院肿瘤中心,广州510507

出　　处：《临床肿瘤学杂志》2013年第3期212-215,共4页Chinese Clinical Oncology

基　　金：中华国际医学交流基金会先声抗肿瘤治疗专项科研基金资助项目(CIMF-F-H001-253)

摘　　要：目的探讨252锎(252Cf)中子射线对宫颈癌组织中胶质瘤相关癌基因蛋白1(Gli-1)和血管内皮生长因子(VEGF)表达的影响。方法选取25例接受252Cf中子腔内放疗联合体外照射的宫颈癌患者的宫颈癌组织,采用免疫组化SP法和免疫印迹法分别检测放疗前后宫颈癌组织中VEGF和Gli-1的表达情况,并分析不同病理参数下放疗前后的VEGF阳性细胞率和Gli-1相对光密度,同时选取5例宫颈炎症组织作对照。结果宫颈癌组织放疗前的VEGF高表达率和Gli-1相对光密度分别为80.0%和0.80±1.73,高于放疗后的44.0%和0.41±0.65及宫颈炎症组织的20.0%和0.20±0.50,差异有统计学意义(P<0.05);不同分期和分化水平宫颈癌组织放疗后的VEGF阳性细胞率、Gli-1相对光密度均低于放疗前(P<0.05)。结论 252Cf中子射线腔内照射可以降低宫颈癌组织中VEGF和Gli-1的表达水平。Objective To investigate the influence of californium-252（252Cf） neutron ray on the expressions of the glioma-as- sociated oncogene homolog 1 （Gli-1） and vascular endothelial growth factor（VEGF） in tumor tissues from cervical cancer patients. Methods The cervical cancer tissues from 25 cervical cancer patients receiving 252Cf neutron ray brachytherapy and external irradia- tion were collected. The expressions of VEGF and Gli-1 before and after radiotherapy in cervical cancer tissues were detected by immn- nohistochemical SP method and western blot, respectively. The VEGF-positive cells and the Gli-1 relative optical density of different pathological parameters before and after radiotherapy were compared. Five cases of cervicitis tissues were chosen as control. Results The high expression rate of VEGF and the relative optical density of Gli-1 in cervical cancer tissues before radiotherapy were 80. 0% and 0. 80 ±1.73, higher than 44. 0% and 0. 41±0. 65 after radiotherapy and 20. 0% and 0. 20 ±0. 50 in cervicitis tissues with signifi- cant differences（ P 〈 0. 05 ）. The VEGF-positive cells and the Gli-1 relative optical density after treatment of different stages and differ- entiation levels were lower than before （ P 〈 0. 05 ）. Conclusion The 252 Cf neutron ray can significantly decrease the expressions of VEGF and Gli-1.

关 键 词：252Cf中子射线 宫颈癌 胶质瘤相关癌基因蛋白1 血管内皮生长因子 

分 类 号：R737.3[医药卫生—肿瘤]