超高效液相色谱快速检测禽蛋类食品中苏丹红(Ⅰ、Ⅱ、Ⅲ、Ⅳ)  

Simultaneous determination of Sudan red(Ⅰ,Ⅱ,Ⅲ,Ⅳ) in egg products by UPLC

作　　者：刘家阳[1] 李敏[1] 贾宏欣[1] 邹淼[1] 

机构地区：[1]辽宁省疾病预防控制中心,沈阳110005

出　　处：《中国卫生检验杂志》2013年第3期599-600,共2页Chinese Journal of Health Laboratory Technology

摘　　要：目的:建立禽蛋类食品中苏丹红(Ⅰ、Ⅱ、Ⅲ、Ⅳ)的超高效液相色谱快速检测方法。方法:采用Xterra BEH C18(2.1×50 mm,1.7μm)色谱柱分离,乙腈-水作为流动相进行梯度洗脱,PDA二极管阵列检测器。结果:苏丹红(Ⅰ、Ⅱ、Ⅲ、Ⅳ)在0.10μg/ml～5.0μg/ml时,浓度与峰面积呈良好的线形关系(r=0.9999),苏丹红(Ⅰ、Ⅱ、Ⅲ、Ⅳ)检出限均为0.010 mg/kg,加标回收率在92.4%～99.0%之间,相对标准偏差RSD在0.63%～1.83%之间。结论:该方法简便、快速、准确,适用于禽蛋类食品中苏丹红(Ⅰ、Ⅱ、Ⅲ、Ⅳ)含量的测定。Objective：A method has been established for the simultaneous determination of Sudan Red （Ⅰ、Ⅱ、Ⅲ、Ⅳ） in egg products by UPLC. Methods： The target analytes were separated on a Xterra BEH C18 （2.1 × 50 mm,1.7μm） with acetonitrile and water as mobile phase for gradient elution, then detected by PDA. Results： When the Sudan Red was at 0.10 μg/ml -5.0 μg/ml , the perfect linearity between concentration and peak area ra- tio was obtained（r = 0. 9999）. The detection limit of glutathione was 0. 010 mg/kg, the standard recovery was be- tween 92.4% ~ 99.0% by adding standard to sample solution and the RSD for the within - run precision was be- tween 0.63% - 1.83%. Conclusion： This method is simple,rapid and accurate,so it can be used for the determi- nation of Sudan Red （ Ⅰ、Ⅱ、Ⅲ、Ⅳ ） in egg products.

关 键 词：超高效液相色谱 禽蛋类食品 苏丹红 

分 类 号：O657.72[理学—分析化学]