反相高效液相色谱法测定奶粉中维生素A和维生素E异构体  

Determination of Vitamin A and the isomers of Vitamin E in milk powder by reversed phase high performance liquid chromatography

作　　者：孙亚兰[1] 李帮锐[2] 黎冠[1] 刘梅芳[3] 王一红[2] 冯家力[2] 

机构地区：[1]湖南省邵阳市疾病预防控制中心,湖南邵阳422000 [2]湖南省疾病预防控制中心,长沙410005 [3]湖南省岳阳市疾病预防控制中心,湖南岳阳414000

出　　处：《中国卫生检验杂志》2013年第3期612-615,共4页Chinese Journal of Health Laboratory Technology

摘　　要：目的:确定了一种简单有效的准确测定维生素A及维生素E异构体的方法。方法:样品经皂化萃取后,用C30柱分离,以乙醇-异丙醇-水为流动相,在波长325 nm检测维生素A,295 nm检测维生素E。采用外标法对视黄醇乙酸酯、α-生育酚、β-生育酚、γ-生育酚、δ-生育酚进行定量分析。结果:各组分线性关系良好,精密度较高,标准皂化回收率和样品加标回收率较高。结论:该方法简便准确,适合于奶粉维生素A和维生素E异构体的定量分析。Objective：To identify a simple and effective method for accurate determination of the vitamin A and vitamin E isomers. Methods： The sample was separated on the Carbon-30 column followed by saponification and extraction. The mobile phase consisted of Ethanol, isopropanol and water. The vitamin A was detected at a wavelength of 325 nm, ritamin E at 295 nm. The retinol acetate, α - tocopherol, β - tocopherol, γ- tocopherol, δ - to- copherol were identified and calculated with external standard method. Results： The correlation coefficients were good, the method suggested the high degree of precision, stability and the repeatability, and the standard saponification recoveries and spiked recoveries. Conclusion： The method is simple and accurate, suitable for quantitative analysis of the vitamin A and vitamin E isomers in milk powder.

关 键 词：反相高效液相色谱法 维生素A 维生素E异构体 定量分析 

分 类 号：O657.72[理学—分析化学]