赤芝子实体中三萜酸类成分及其抗人乳腺癌SKBR3细胞增殖活性  

Triterpenoid acids from sporophore of Ganoderma lucidum and their inhibition on proliferatin of SKBR3 human breast cancer cells

作　　者：董虹玲[1,2] 赵娜夏[1] 商倩[1] 夏广萍[1] 白秀秀[1,2] 邵泽艳[1,2] 韩英梅[1] 

机构地区：[1]天津药物研究院天津市新药设计与发现重点实验室,天津300193 [2]天津中医药大学,天津300193

出　　处：《现代药物与临床》2013年第2期132-137,共6页Drugs & Clinic

基　　金：国家重大新药创制专项(2011ZX09-401-009)

摘　　要：目的研究赤芝Ganoderma lucidum子实体中三萜酸类成分及其抗肿瘤活性。方法用硅胶柱色谱、反相ODS柱色谱、反相HPLC等方法分离得到13个化合物,利用波谱学方法鉴定它们的化学结构,并进一步确定各化合物对乳腺癌SKBR3细胞的生物活性。结果分离得到13个三萜酸类的化合物,分别鉴定为灵芝烯酸C(1)、灵芝酸C2(2)、灵赤酸A(3)、灵芝酸G(4)、灵芝烯酸B(5)、灵芝酸B(6)、灵芝酸H(7)、灵芝烯酸A(8)、灵芝酸I(9)、灵芝酸A(10)、灵芝烯酸K(11)、灵芝烯酸D(12)、灵芝酸D(13)。化合物6在20μmol/L浓度下对人乳腺癌细胞SK-BR-3的抑制率为40.3%。结论化合物11是首次从该植物中分离得到的,化合物6对人乳腺癌细胞SK-BR-3显示了一定的抑制活性,其他化合物在该浓度下未显示活性。Objective To study the triterpenoid acids in sporophore of Ganoderma lucidum and their antitumor activities.Methods The compounds were isolated by the silica gel,ODS column chromatography and HPLC techniques,then their structures were identified by their physicochemical characteristics and spectral features.The inhibitory activity of all compounds against SKBR3 human breast cancer cells was assessed.Results Thirteen compounds were isolated and identified as ganoderenic acid C（1）,ganoderic acid C2（2）,ganolucid acid A（3）,ganoderic acid G（4）,ganoderenic acid B（5）,ganoderic acid B（6）,ganoderic acid H（7）,ganoderenic acid A（8）,ganoderic acid I（9）,ganoderic acid A（10）,ganoderenic acid K（11）,ganoderenic acid D（12）,and ganoderic acid D（13）.Compound 6（20 μmol/L） exhibited an inhibitory rate of 40.3% against SKBR3 human breast cancer cells.Conclusions Compound 11 is isolated from this plant for the first time.Compound 6 exhibits the inhibition against SKBR3 human breast cancer cells at 20 μmol/L,but other compounds have no activities at the same concentration.

关 键 词：赤芝 子实体 三萜酸 乳腺癌 

分 类 号：R284.1[医药卫生—中药学]