三氧化二砷与顺铂联合诱导人卵巢癌HO8910细胞凋亡的初步研究  

Studies on Arsenic trioxide and Cisplatin induces apoptosis in Ovarian Cancer cell lines HO8910

作　　者：高洪泉[1] 张爱清[1] 田忠富[1] 

机构地区：[1]厦门医学高等专科学校,361008

出　　处：《中国实用医药》2013年第6期250-252,共3页China Practical Medicine

基　　金：福建省教育厅基金资助项目(项目编号:2011);厦门医学高等专科学校基金项目(项目编号:2009)

摘　　要：目的探讨三氧化二砷与顺铂联合对体外培养卵巢癌细胞株HO8910细胞增殖、细胞周期调控及细胞凋亡影响。方法用三氧化二砷与顺铂联合处理HO8910细胞,采用MTT法检测药物对细胞的抑制作用,倒置显微镜观察细胞形态学改变;琼脂糖凝胶电泳观察DNA降解,以及应用流式细胞仪观察细胞凋亡过程中细胞周期的变化。结果在三氧化二砷与顺铂联合作用下,HO8910细胞呈凋亡改变,DNA琼脂糖凝胶电泳呈典型的凋亡特征。细胞凋亡的同时,细胞周期发生特定的改变。结论三氧化二砷与顺铂联合能抑制卵巢癌细胞增殖,能诱导卵巢癌细胞凋亡。Objective The current study was designed to investigate whether Arsenic trioxide and Cisplatin can induces apoptosis in Ovarian Cancer cell.Methods Cell proliferation was assessed by MTT assays.Morphological changes of apoptosis were observed with invert microscope.DNA ladder and cell cycle were examined by DNA agarose gel eletronphores and PI fluorescence flow cytometry（FCM）.Results Under the influence of Arsenic trioxide and Cisplatin,HO8910 cell exhibited changes of apoptosis both in morphology and in the characteristics of electrophoresis.Meanwhile the cell cycle also showed special change.Conclusion Arsenic trioxide and Cisplatin can inhibit cell proliferation,Arsenic trioxide and Cisplatin can induces apoptosis in Ovarian Cancer cell.

关 键 词：三氧化二砷(As2O3) 顺铂(DDP) 细胞凋亡 卵巢癌细胞株 

分 类 号：R737.31[医药卫生—肿瘤]