Antitumor Effects of Newcastle Disease Virus Hemagglutinin-neuraminidase Used as a Molecular Adjuvant  

作　　者：JI Hui-fan CHI Bao-rong HE Dong-yun LI Chang HU Ning-ning WANG Kai SHENG Yuan WANG Hong-yu JIN Ning-yi 

机构地区：[1]Department of Gastroenterology, the First Hospital of Jilin University, Changchun 130021, P. R. China [2]Institute of Military Veterinary, Academy of Military Medical Sciences of PLA, Changchun 130122, P. R. China, [3]Key Laboratory of Pathobiology, Ministry of Education, Jilin University, Changchun 130021, P. R. China

出　　处：《Chemical Research in Chinese Universities》2013年第2期270-274,共5页高等学校化学研究（英文版）

基　　金：Supported by the National Science and Technology Major Projects for Major New Drugs Innovation and Development of China（No.2010ZX09401-305-14）, the National Natural Science Foundation of China（Nos.81072210, 81101140） and the Key Technologies R＆D Program of Jilin Province of China（Nos. 10ZDGG007, 201015166, 201101066）.

摘　　要：Gene therapy is a potentially powerful tool used in cancer therapy. The strength of immune responses in- duced by some strategies is usually low, therefore, the development of agents capable of enhancing these responses is highlighted. The authors investigated the potential of an approach based on the hemagglutinin-neuraminidase（HN） of Newcastle disease virus（NDV） as a potential immune adjuvant. It was found that recombinant adenovirus（Ad） in- fected SGC7901 cells expressing HN exhibited both hemagglutinin（HA） and neuraminidase（NA） activities. It was demonstrated that administration of HN induced higher levels of the effector cytokines TNF-a, IFN-a and IFN-y and increased natural killer（NK） cell activity. Based on the therapeutic tumor model, the results show that the administra- tion of HN with Apoptin led to improved survival and tumor suppression. In conclusion, this study indicates that HN stimulates innate immune responses to make the activity of NK cells increased, which highlights the potential adju- vant activity of HN in cancer gene therapy.Gene therapy is a potentially powerful tool used in cancer therapy. The strength of immune responses in- duced by some strategies is usually low, therefore, the development of agents capable of enhancing these responses is highlighted. The authors investigated the potential of an approach based on the hemagglutinin-neuraminidase（HN） of Newcastle disease virus（NDV） as a potential immune adjuvant. It was found that recombinant adenovirus（Ad） in- fected SGC7901 cells expressing HN exhibited both hemagglutinin（HA） and neuraminidase（NA） activities. It was demonstrated that administration of HN induced higher levels of the effector cytokines TNF-a, IFN-a and IFN-y and increased natural killer（NK） cell activity. Based on the therapeutic tumor model, the results show that the administra- tion of HN with Apoptin led to improved survival and tumor suppression. In conclusion, this study indicates that HN stimulates innate immune responses to make the activity of NK cells increased, which highlights the potential adju- vant activity of HN in cancer gene therapy.

关 键 词：Hemagglutinin-neuraminidase（HN） ADJUVANT Innate immunity Natural killer cell Antitumor immunity 

分 类 号：S852.659.5[农业科学—基础兽医学] S852.4[农业科学—兽医学]