Optimization of the Technique for Liquid Culture of Pleurotus cornucopiae Seeds  

姬菇液体菌种生产工艺优化(英文)

作　　者：温以斌 吴义飞[2,3] 王多明[1] 李冠喜[1,2,3] 刘辉[1] 刘耀鸿[1] 

机构地区：[1]连云港市农业科学院,江苏连云港222006 [2]南京林业大学森林资源与环境学院,江苏南京210037 [3]江苏省入侵有害生物预防与控制重点实验室,江苏南京210037

出　　处：《Agricultural Science & Technology》2013年第3期507-511,共5页农业科学与技术（英文版）

基　　金：Supported by the Agricultural "Three Terms" Program in Jiangsu Province (sx (2009) 133);the Plan Program for Demonstration of Scientific and Technological Achievements in Agriculture of Lianyungang City(SF0902)~~

摘　　要：[Objective] This study aimed to optimize the conditions for liquid culture of P. comucopiaeseeds. [Method] ＂P. cornucopiae 2＇ selected by our laboratory was used as the experimental strain in this study. Single factor experiment in conjunction with Plackett-Burman （PB） design as well as Response Surface Methodology（RSM） based on Central Composite Design （CCD） were used to screen and optimize the eight factors influencing the technique for liquid culture of P. cornucopiae seeds. [Result] Firstly, the eight factors influencing the biomass of P. cornucopiae seeds in liquid culture were confirmed by means of single factor experiment; secondly, through Plackett-Burman experiment design, three main factors influencing the biomass of P. cornucopiae seeds in liquid culture were screened, namely the amount of added mannitol, the initial pH and culture temperature; lastly, the second order model, that is Y=-70.72＋2.44X3 ＋2.67X7 ＋3.72X8 ＋0.08X3 X7 -0.01X7 X8-0.05X32- 0.34X72-0.08X82, was obtained through Central Composite Design （CCD） along with Response Surface Methodology （RSM）. [Conclusion] The optimal conditions for the production of P. cornucopiae seeds in liquid culture were as follows： the amount of added mannitol of 28.17 g/L, the initial pH controlled at 5.90, 250 ml erlenmeyer flask filled with 100 ml of liquid medium, inoculum size of 5%（v/v）, rotation speed of 150 r/min, and gyratory culture at 23.97℃ for 5 days.[目的]对姬菇液体菌种培养条件进行优化。[方法]以该实验室筛选的品种姬菇2号为研究对象,采用单因素试验方法结合Plackett-Burman(PB)试验及利用Central Composite Design(CCD)试验设计的响应面分析法(Response Surface Methodology,RSM)对姬菇液体菌种生产工艺的8个参数进行筛选优化。[结果]通过单因素试验,确定了影响姬菇液体菌种生物量的8个因素,通过PB试验,筛选出3个影响姬菇液体菌种生物量的3主要因子为甘露醇的加入量、初始PH值和培养温度,通过CCD设计及响应面法优化分析得到影响姬菇液体菌种生物量的二阶模型:Y=-70.72+2.44X3+2.67X7+3.72X8+0.08X3X7-0.01X7X8-0.05X32-0.34X72-0.08X82。[结论]姬菇液体菌种生产的最优操作条件确定为:姬菇液体菌种培养基中甘露醇加入量为28.17g/L,初始pH值控制在5.90,250ml三角瓶装液量100ml,接种量5%(V/V),摇床转速150r/min,23.97℃振荡培养5d。

关 键 词：Pleurotus cornucopiae BIOMASS Influencing factors OPTIMIZATION 

分 类 号：S646[农业科学—蔬菜学]