NRP-1在大鼠肝纤维化组织中的表达及其与细胞外基质成分的相关性  

Correlation between expression of neuropilin-1 and that of extracellular matrix components in liver fibrosis in rats

作　　者：魏海艳[1] 潘亮[2] 黄华[2] 肖明兵[2] 陆翠华[2] 

机构地区：[1]江苏省阜宁县人民医院消化内科,江苏省盐城市224400 [2]南通大学附属医院消化内科,江苏省南通市226001

出　　处：《世界华人消化杂志》2013年第8期685-689,共5页World Chinese Journal of Digestology

摘　　要：目的:研究神经纤毛蛋白1(neuropilin-1,NRP-1)在肝纤维化组织中的动态表达及其与细胞外基质成分的相关性.方法:将动物分为正常对照组和模型组,用CCl4复制肝纤维化模型,采用RT-PCR和免疫组织化学方法联合检测NRP-1、Ⅰ、Ⅲ型胶原和α-平滑肌肌动蛋白(α smooth muscle actin,α-SMA)在肝纤维化组织中的表达并进行统计学分析.结果:随着肝纤维化程度的加重,NRP-1、α-SMA和Ⅰ、Ⅲ型胶原的表达均逐步增加.在2wk时NRP-1与Ⅰ、Ⅲ型胶原的相关性不明显,但与α-SMA呈显著相关,其相关系数为0.621;在4wk时与Ⅰ、Ⅲ型胶原和α-SMA的相关系数分别为0.645、0.530和0.690;在6wk时与Ⅰ、Ⅲ型胶原和?-SMA的相关系数分别为0.834、0.613和0.820.结论:NRP-1在肝纤维化的发生发展中起重要作用.AIM： To investigate the expression of neuropi- lin-1 （NRP-1） in liver fibrosis in rats and to ana- lyze its correlation with the expression of extra- cellular matrix components. METHODS： Hepatic fibrosis was induced in rats using carbon tetrachloride. The mRNA and pro- tein expression of NRP-1, collagen I, collagen III and α smooth muscle actin （α-SMA） in he- patic tissue of the rats was examined by reverse transcription-polymerase chain reaction （RT- PCR） and immunohistochemistry, respectively. Correlation between the expression of NRP-1 and that of collagen I, collagen III and α-SMA was analyzed. RESULTS： The expression of NRP-1, α-SMA, collagen I, and collagen III proteins was low in the liver tissue of normal rats. In rats with exper- imental liver fibrosis, the expression of NRP-1, α-SMA, collagen I, and collagen III proteins in- creased with the progression of hepatic fibrosis. At weeks 4 and 6, the expression of the above four proteins were significantly higher in liver fi- brosis than in normal hepatic tissue （all P 〈 0.05）. NRP-1 mRNA expression in fibrotic liver tissue was significantly higher than that in normal hepatic tissue （P 〈 0.05）. Expression of α-SMA, collagen I, and collagen III mRNAs was low in normal liver tissue but was significantly in- creased at weeks 2, 4, and 6 after injection of carbon tetrachloride. The expression of NRP-1, α-SMA, collagen I, and collagen III mRNAs was gradually increased with the progression of hepatic fibrosis. NRP-1 expression had no appar- ent correlation with collagen I and collagen III expression at week 2, but showed a significant correlation at weeks 4 （r = 0.645, 0.530） and 6 （r = 0.834, 0.613）. NRP-1 expression had a significant correlation with （x-SMA expression during the whole process of hepatic fibrosis （r = 0.621, 0.690, 0.820, all P 〈 0.05）. CONCLUSION： NRP-1 is overexpressed in he- patic fibrosis, which correlates with the expres- sion of collagen I and collagen III.

关 键 词：肝纤维化 神经纤毛蛋白1 肝星状细胞 

分 类 号：R575.2[医药卫生—消化系统]