中医解毒法优化干预对BALB/c结肠癌小鼠CEA、sCD44的实验研究  

The Experiment Research on Optimization Intervention of Disintoxication on BALB/c Colon Cancer Mice in CEA、sCD44

作　　者：阮善明[1] 沈敏鹤[1] 王益[2] 林红[2] 郑丽萍[2] 李梦婷[2] 张恺[2] 

机构地区：[1]浙江中医药大学附属第一医院肿瘤中心,杭州310006 [2]浙江中医药大学,杭州310053

出　　处：《世界中医药》2013年第3期313-317,共5页World Chinese Medicine

基　　金：国家公益性行业科研专项项目(编号:200807018);浙江省教育厅项目(编号:Y201016272)

摘　　要：目的:探索中医解毒法协同化疗在结肠癌辅助治疗阶段的优化治疗模式,并初步探讨作用机制。方法:1)皮下接种CT-26细胞于50只BALB/c小鼠建立移植瘤模型,分为5组:提前干预组10只、延后干预组10只、单纯中药组10只、单纯化疗组10只、模型对照组10只。同时增设阴性对照组10只。2)5组移植瘤模型小鼠以及1组阴性对照组小鼠,治疗期间定期监测体重及瘤体变化。分组取血,分离血清,取瘤称瘤重,计算抑瘤率。3)ELISA法检测各组血清CEA、sCD44含量。结果:1)抑瘤率:两药合用组的抑瘤率约50%～60%,单纯化疗组约40%,单纯中药组约20%。抑瘤率从高到低依次为提前干预组、延后干预组、单纯化疗组、单纯中药组。2)小鼠去瘤体重变化:治疗末提前干预组、单纯中药组小鼠去瘤体重较治疗前增加,单纯化疗组、延后干预组、模型对照组治疗后体重下降。3)ELISA结果显示:各治疗组CEA、sCD44含量较模型对照组均有下降,两药合用组较单药治疗组下降明显。提前干预组、延后干预组、单纯化疗组的CEA、sCD44含量与模型对照组比较差异有统计学意义(P<0.05)。结论:中医解毒法协同化疗能抑制肿瘤的作用机制可能与降低血清CEA、sCD44表达有关。Objective： To investigate the optimum combination of Disintoxication and chemotherapy in auxiliary treatment phase on colon cancer xenografts in mice, elucidate the potential mechanism of Chinese herbal for adjuvant chemotherapy. Methods： 1 ） Colon cancer cell line CT-26 was injected subcutaneously on 50 BALB/c mice to establish colon cancer xenografts model, they were randomly divided into 5 groups： 10 BALB/C mice in pre-intervention group,10 in post-intervention group,lO in Herbal only Herbal group,10 in Chemo- therapy only group,lO BALB/C mice in model control, the other 10 mice were used as negative control group. 2） Body weight and tumor volume were measured during the treatment in the BALB/c mice from each groups and negative control group. Harvesting blood from dif- ferent groups, separating serum, weighting tumor weight and calculating anti-tumor rate. 3 ） Detecting content of CEA,sCD44 in serum from different groups by ELISA. Results：l ） Tumor inhibition rate： The anti-tumor rate was 50%-60% in optimum combination groups, while 40% in chemotherapy group and 20% in Herbal only group, in sequence of Pre- intervention group 〉 Post- intervention group 〉 Chemotherapy only group 〉 Herbal only group. 2） Change of mice weight without tumor weight： Compared with the weight before the treatment, mice weight increased after treatment in Pre- intervention group and Herbal only group. While the result is opposite in chemo- therapy group ,post-intervention group and model control group. 3 ） Serum level of CEA and sCDd-4 ： Compared with model control group, the content of CEA ,sCD44 decreased in treatment groups, while with dramatic decline in two therapy combination group than single drug treatment group. Compared with model control group, the content of CEA,sCD44 in pre-intervention group,post-intervention group and chemotherapy group had statistical significance （ P 〈 0.05 ）. Conclusion： The mechanism of the combination of detoxicating therapy and chemotherapy inhibits tumor p

关 键 词：虎杖根 水杨梅根 藤梨根 结肠癌 抑瘤率 

分 类 号：R57[医药卫生—消化系统]