水稻糙米蛋白质和粗脂肪含量的QTLs分析  

QTLs Mapping of Protein Content and Crude Fat Content in Rice

作　　者：黄覃 于波 NASSIROU Tondi-yacouba 高冠军[1] 张庆路[1] 何予卿[1] 

机构地区：[1]华中农业大学作物遗传改良国家重点实验室,武汉430070

出　　处：《湖北农业科学》2012年第21期4709-4713,共5页Hubei Agricultural Sciences

基　　金：国家自然科学基金项目(30971550);国家"863"计划项目(2012AA101102);植物转基因专项(2011ZX08001-002);现代农业水稻产业技术体系项目(CARS-01-03)

摘　　要：蛋白质和粗脂肪含量是评价稻米营养品质的重要指标,控制水稻(Oryza sativa L.)糙米蛋白质及脂肪含量的基因位点是数量性状,检测水稻糙米蛋白质及脂肪含量的数量性状位点(QTL)对于水稻品质遗传育种具有重要的意义。通过明恢63和优质泰国香米KDML105两个籼稻品种为亲本杂交的重组自交系(Recombinant inbred line,RIL)群体构建了包含113个简单重复序列标记(SSR)的遗传连锁图谱,对2009、2010年群体的蛋白质和粗脂肪含量进行了QTL定位。2009年检测到5个QTLs,其中蛋白质含量检测到2个QTLs,单个QTL贡献率分为别为5.44%和5.52%;粗脂肪含量检测到3个QTLs,单个QTL贡献率为5.42%～7.30%。2010年蛋白质含量检测到3个QTLs,单个QTL可解释表型变异为6.24%～20.75%;未检测到粗脂肪含量QTL。此外,还检测到14对粗脂肪含量和8对蛋白质含量的上位性QTLs。Protein content and fat content in rice are important traits for evaluation of nutrient quality which was controlled by polygenes.Recombinant inbred lines（RIL） population derived from a cross between two indica rice varieties,Minghui63 and KDML105,were used to construct a linkage map consisting of 113 simple sequence repeat（SSR） markers.Quantitative trait loci（QTLs） mapping for controlling grain fat and protein content were conducted in 2009 and 2010.5 QTLs were identified in 2009,including 2 QTLs for grain protein content and 3 QTLs for grain fat content;the phenotypic variation explained by individual QTL ranged from 5.44%～5.52% in protein content and 5.42%～7.30% in fat content.In 2010,3 QTLs were detected for grain protein content and the phenotypic variation explained by individual QTL ranged from 6.24%～20.75%.

关 键 词：水稻(Oryza sativaL ) 蛋白质 粗脂肪 重组自交系 数量性状位点 

分 类 号：S511.21[农业科学—作物学] Q943.2[生物学—植物学]