集胞藻PCC6803基因ssl1690缺失突变株的构建  

Construction of Deletion Mutant Strain of Gene ssl1690 from Synechocystis sp. PCC6803

作　　者：陈思礼[1] 镇慧[1] 

机构地区：[1]中南民族大学生命科学学院,武汉430074

出　　处：《中南民族大学学报（自然科学版）》2013年第1期28-31,共4页Journal of South-Central University for Nationalities：Natural Science Edition

基　　金：国家自然科学基金资助项目(31001099/C190101);中央高校自然科学基金资助项目(CJS12003);中南民族大学微生物与生物转化重点实验室资助项目(XJS09002)

摘　　要：使用BLAST软件对聚球藻PCC7002的裂合酶基因cpcT进行了同源搜索分析,在集胞藻PCC6803中获取了相似程度达68%的同源基因ssl1690.为进一步探讨ssl1690功能,构建了同源缺失突变载体,将其转入蓝藻细胞中,筛选得到基因缺失突变株,并将基因缺失突变株培养于BG11液体培养基中,观察了其生长情况和表型变化.结果表明:基因缺失突变株与野生株相比,生长有所延迟,有漂白现象.通过分离和比较SDS-PAGE电泳突变株与野生株的藻胆体蛋白,发现缺失株存在藻胆体组分蛋白的缺失.故认为集胞藻PCC6803 ssl1690基因功能与光合作用有关,其所编码的蛋白对藻蓝蛋白正常的体内生物合成具有重要作用.Gene ssl1690 was obtained by BLAST comparison from Synechocystis sp.PCC6803 that is 68% homologous to lyase gene cpcT.To study the biological function of ssl1690,homologous knock-out mutation vector was constructed and transferred into Synechocystis sp.PCC6803.Then the ssl1690 knock-out mutant strain was screened out through the resistance molecular marker and the growth condition and cultivating phenotype changes of mutant strain contrasted with wild strain was observed.The results indicated that deletion mutant strain grew more slowly than wild type strain and displayed bleaching phenomenon.Isolatation and SDS-PAGE electrophoresis of phycobiliprotein in wild type and deletion mutant showed that deletion mutant exhibited constitutive loss of phycobilisomes.All above suggested ssl1690 gene of Synechocystissp PCC6803 was related to photosynthesis and encoded protein may play an important role in normal biosynthesis in phycocyanin in vivo.

关 键 词：集胞藻PCC6803 同源重组 缺失突变 漂白现象 

分 类 号：Q784[生物学—分子生物学]