Potent T cell Responses Induced by Single DNA Vaccine Boosted with Recombinant Vaccinia Vaccine  

作　　者：Lianxing Liu Chao Qiu Yang Huang Jianqing Xu Yiming Shao 

机构地区：[1]State Key Laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of Sciences [2]State Key Laboratory for Infectious Diseases Prevention and Control, National Center for AIDS/STD Control and Prevention, Chinese Center for Disease Control and Prevention [3]Science Research Department, Shanghai Public Health Clinical Center, Public Health Clinical Center affiliated to Fudan University

出　　处：《Virologica Sinica》2013年第2期109-115,共7页中国病毒学（英文版）

基　　金：supported by the China Comprehensive Integrated Programs for Research on AIDS(CIPRA, U19AI51915);by the National Key Projects on Major Infectious Diseases (Grant No. 2008ZX10001-010,2012ZX10001-008);by the National Natural Science Foundation of China (31000413)

摘　　要：Plasmid DNA, an effective vaccine vector, can induce both cellular and humoral immune responses. However, plasmid DNA raises issues concerning potential genomic integration after injection. This issue should be considered in preclinical studies. Tiantan vaccinia virus (TV) has been most widely utilized in eradicating smallpox in China. This virus has also been considered as a successful vaccine vector against a few infectious diseases. Potent T cell responses through T-cell receptor (TCR) could be induced by three injections of the DNA prime vaccine followed by a single injection of recombinant vaccinia vaccine. To develop a safer immunization strategy, a single DNA prime followed by a single recombinant Tiantan vaccinia (rTV) AIDS vaccine was used to immunize mice. Our data demonstrated that one DNA prime/rTV boost regimen induced mature TCR activation with high functional avidity, preferential T cell Vβ receptor usage and high sensitivity to anti-CD3 antibody stimulation. No differences in T cell responses were observed among one, two or three DNA prime/rTV boost regimens. This study shows that one DNA prime/rTV boost regimen is sufficient to induce potent T cell responses against HIV.Plasmid DNA, an effective vaccine vector, can induce both cellular and humoral immune responses. However, plasmid DNA raises issues concerning potential genomic integration after injection. This issue should be considered in preclinical studies. Tiantan vaccinia virus （TV） has been most widely utilized in eradicating smallpox in China. This virus has also been considered as a successful vaccine vector against a few infectious diseases, Potent T cell responses through T-cell receptor （TCR） could be induced by three injections of the DNA prime vaccine followed by a single injection of recombinant vaccinia vaccine. To develop a safer immunization strategy, a single DNA prime followed by a single recombinant Tiantan vaccinia （rTV） AIDS vaccine was used to immunize mice. Our data demonstrated that one DNA prime/rTV boost regimen induced mature TCR activation with high functional avidity, preferential T cell Vβ receptor usage and high sensitivity to anti-CD3 antibody stimulation. No differences in T cell responses were observed among one, two or three DNA prime/rTV boost regimens. This study shows that one DNA prime/rTV boost regimen is sufficient to induce potent T cell responses against HIV.

关 键 词：HIV VACCINE T cell responses Prime-boost regimen 

分 类 号：R392[医药卫生—免疫学]