Molecular Characterization of China Human Rabies Vaccine Strains  

作　　者：Xiaoyan Tao Na Han Zhenyang Guo Qing Tang Simon Rayner Guodong Liang 

机构地区：[1]Key Laboratory of Medical Virology, Ministry of Health, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention [2]Key Laboratory of Agricultural and Environmental Microbiology, Wuhan Institute of Virology, Chinese Academy of Sciences [3]State Key Laboratory for Infectious Disease Prevention and Control, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention

出　　处：《Virologica Sinica》2013年第2期116-123,共8页中国病毒学（英文版）

基　　金：supported by the National Department Public Benefit Research Foundation (201103032)

摘　　要：To understand the molecular characteristics of China human rabies vaccine strains, we report the full-length genome of the aG strain and present a comprehensive analysis of this strain and almost all available lyssavirus genomes （58 strains） from GenBank （as of Jan 6, 2011）. It is generally considered that the G protein plays a predominant role in determining the pathogenicity of the virus, to this end we predicted the tertiary structure of the G protein of aG strain, CTN 181 strain and wild type strain HN 10 based on the crystal structure of Vesicular stomatitis virus （VSV） G. The predicted RABV G structure has a similar topology to VSV G and the ectodomain can be divided into 4 distinct domains DI - DIV. By mapping the characterized mutations to this structure between China vaccine strains and their close street strains, we speculate that the G303（P-H） mutations of CTN181 and HN10 causing D II 3D change may be associated with the attenuated virulence in both strains. Specifically, the two signature mutations （G165P and G231P） in the aG strain are withinβsheets, suggesting that both sites are of structural importance.To understand the molecular characteristics of China human rabies vaccine strains, we report the full-length genome of the aG strain and present a comprehensive analysis of this strain and almost all available lyssavirus genomes (58 strains) from GenBank (as of Jan 6, 2011). It is generally considered that the G protein plays a predominant role in determining the pathogenicity of the virus, to this end we predicted the tertiary structure of the G protein of aG strain, CTN181 strain and wild type strain HN10 based on the crystal structure of Vesicular stomatitis virus (VSV) G. The predicted RABV G structure has a similar topology to VSV G and the ectodomain can be divided into 4 distinct domains DI-DIV. By mapping the characterized mutations to this structure between China vaccine strains and their close street strains, we speculate that the G303(P-H) mutations of CTN181 and HN10 causing DⅡ 3D change may be associated with the attenuated virulence in both strains. Specifically, the two signature mutations (G165P and G231P) in the aG strain are withinβsheets, suggesting that both sites are of structural importance.

关 键 词：Rabies virus LYSSAVIRUS GENOME GLYCOPROTEIN 

分 类 号：R392[医药卫生—免疫学]