Df(3R)Esp13/TM6C片段RNA干扰不同基因对黑腹果蝇睡眠时间的影响  

INTERFERENCE OF DIFFERENT GENES IN Df(3R)Esp13/TM6C RNA FRAGMENT AFFECT SLEEP DURATION OF DROSOPHILA MELANOGASTER

作　　者：张迪[1] 张可兴[1] 黄莉莉[1] 李廷利[1] 

机构地区：[1]黑龙江中医药大学,哈尔滨150040

出　　处：《中国药物依赖性杂志》2013年第2期112-115,共4页Chinese Journal of Drug Dependence

基　　金：黑龙江省研究生创新科研项目(YJSCX2012-355HLJ);教育部春晖计划(Z2007-1-15003);国家自然科学基金青年基金(30801528)

摘　　要：目的:寻找影响果蝇睡眠时间的基因。方法:选择Df(3R)Esp13/TM6C片段RNA干扰不同基因的7日龄黑腹果蝇与野生型CS品系7日龄黑腹果蝇为研究对象,利用果蝇活动监测器系统(Drosophila Activity Monitor System,DAMS),记录果蝇的睡眠时间,累计计算24 h内果蝇睡眠时间,将测得的各品系果蝇睡眠时间进行对比分析。结果:RNA干扰dys,exo84和Nf1基因的黑腹果蝇与野生型CS品系黑腹果蝇相比较具有极显著性差异(P<0.001)。结论:Nf1,exo84和gro基因与睡眠有关。Objective ：To find the proper genes that affect sleep duration. Methods：Different genes of RNA interference in Df（3R）Esp13/TM6C fragment of the 7 -day old Drosophila melanogaster and CS strains of D. melanogaster were used in the research. Drosophila Activity Monitor System（ DAMS）was used to record the Drosophila＇ s total sleep duration within 24 hours. The data was analyzed according to different kinds of strains.Results： Compared with the prosopon of wild CS strains, the total sleep duration of genes of RNA inference dys, exo84 and Nfl were significantly different（P 〈0.001 ）. Conclusion：The genes of Nil,exo84 and gro are related to the sleep duration of D. melanogaster.

关 键 词：黑腹果蝇 睡眠 基因 突变体 果蝇活动监测系统 

分 类 号：R394[医药卫生—医学遗传学]