响应曲面分析法优化油茶籽中总黄酮提取工艺  

Optimization of Extraction Technology for Total Flavonoids from Camellia oleifera Seeds by Response Surface Methodolgy

作　　者：陈诗强[1] 林江萧[2] 陈剑锋[2] 刘国敏 龚林光[2] 梁一池[1] 

机构地区：[1]福建中医药大学药学院,福州350122 [2]福州大学天然产物与中药现代化研究所,福州350108 [3]福建省闽侯桐口国有林场,福州352101

出　　处：《中国实验方剂学杂志》2013年第8期26-29,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：福建省科技厅重点项目(2011N1007);福建省自然科学基金计划项目(2011J05073)

摘　　要：目的:优化油茶籽中总黄酮的乙醇提取工艺。方法:以提取温度、提取时间、乙醇体积分数、料液比为自变量,应用Box-Behnken中心组合设计建立数学模型,以总黄酮提取率为响应值作响应面和等高线,采用响应面法优化油茶籽中总黄酮的提取工艺。结果:最佳提取工艺条件为温度66℃,70%乙醇提取1.3 h,料液比1∶22;油茶籽总黄酮提取率1.87%,与理论值(1.89%)接近。结论:该优选工艺稳定可靠,为油茶资源的合理利用提供参考。Objective：To optimize extraction technology of total flavonoids from Camellia oleifera seeds.Method：With extraction temperature,extraction time,ethanol concentration and solid-liquid ratio as independent variables,Box-Behnken central composite design was used to establish mathematical model,response surface and contour were made with yield of total flavonoids as response value,response surface methodology was adopted to optimize extraction technology of total flavonoids from C.oleifera seeds.Result：Optimum extraction technology conditions were as followings：extraction temperature 66 ℃,ethanol concentration 70%,extracting time 1.3 h,solid-liquid ratio of 1∶22.Under these conditions,yield of total flavonoids from C.oleifera seeds was 1.87%,which was close to the theoretical value of 1.89%.Conclusion：This optimized technology was stable and reliable,and it could provide reference for rational utilization of resources of C.oleifera.

关 键 词：油茶籽 总黄酮 提取 优化 响应曲面分析法 

分 类 号：R283.6[医药卫生—中药学]