不同方法提取木薯茎总RNA效果的比较研究  

Comparative Study on Different Extraction Methods of Total RNA from Stem of Manihot esculenta Crantz

作　　者：曾文丹[1,2] 罗兴录[1] 袁圣勇[1] 杨鑫[1] 郭雅静[1,2] 

机构地区：[1]广西大学农学院,广西南宁530005 [2]广西作物遗传改良生物技术重点开放实验室,广西南宁530007

出　　处：《北方园艺》2013年第8期103-106,共4页Northern Horticulture

基　　金：国家"973"计划资助项目(2010CB126601);广西自然科学基金重点资助项目(2010GXNSFD013025);南宁市科技攻关计划资助项目(201109044B)

摘　　要：以木薯为试材,采用异硫氰酸胍法、热硼酸盐法、Trizol法、TransZol Plant试剂盒法4种不同方法分别提取木薯茎中总RNA,并对其提取效果进行了比较分析,探讨木薯总RNA的有效提取方法。结果表明:除了热硼酸盐法不能有效地提取木薯茎中总RNA以外,其余3种方法均能有效地提取木薯茎中总RNA。其中异硫氰酸胍法提取效果最好,不仅提取耗时最短,仅为1.5h,而且提取的总RNA质量和纯度相对最好。该方法是一种适合于木薯茎中总RNA提取的简单高效的方法。Taking Manihot esculenta Crantz as material,the effects of four different extraction methods（Guanidinium isothiocyanate method,Thermal Borate method,Trizol and TransZol Plant） of total RNA from stems of Manihot esculenta Crantz were studied,in order to obtain the efficient method for RNA extraction.The results showed that total RNA of Manihot esculenta Crantz can＇t be extracted using the Thermal Borate method,and total cassava stems RNA can be extracted effectively using the other three methods.The time（only 1.5 h） and quality of RNA extracted by Guanidinium isothiocyanate method were the best,which was a kind of simple and efficient method suitable for the extraction of total RNA from the stems of Manihot esculenta Crantz.

关 键 词：木薯 总RNA 不同提取方法 提取效果 

分 类 号：S533[农业科学—作物学]