北虫草菌丝体诱变及优良菌株的筛选  

Mutagenesis of Mycelial of Cordyceps militaris and Selection of Fine Strains

作　　者：周晓东[1] 李明[1] 田景花[1] 李守勉[1] 孙雅洁[1] 刘俊波[1] 

机构地区：[1]河北农业大学园艺学院,河北保定071001

出　　处：《北方园艺》2013年第8期154-156,共3页Northern Horticulture

摘　　要：以北虫草‘CM016’为出发菌株,将液体培养的菌丝体片段用生理盐水稀释104倍,在20W紫外灯下32cm处照射100s,研究了紫外线诱变对北虫草优良菌株筛选的影响。结果表明:经紫外线诱变共获得了31个菌丝体长势较好的菌株;拮抗试验表明,其中15株诱变菌株与出发菌株存在拮抗线,对其进一步采用脂酶同工酶电泳鉴定亲缘关系,筛选出了与出发菌株有明显差异的诱变新菌株11个,将其进行出草试验及统计产量和虫草素含量测定,有8个菌株具有出草能力,其中C16菌株子实体产量极显著高于出发菌株,C6菌株子实体虫草素的含量极显著高于出发菌株。Taking Cordyceps militaris＇CM106＇as material,the fragments from the mycelia of C.militaris ＇CM016＇ were diluted to 104 times by using physiological saline cultured in liquid medium.Under a 20 W UV lamp,the fragments were mutated for 100 seconds with a distance of 32 cm.The effect of mutagenesis on selection of fine strains were studied.The results showed that 31 C.militaris strains whose mycelia had better morphological characteristics were obtained.Somatic incompatibility analysis indicated that 15 mutagenic C.militaris strains of all had antagonistic lines with the original strain.Further isozyme analysis showed that 11 of the 15 strains above had obvious differences with the original strain in genetic relationships.Of the 11 mutagenic strains above,8 were able to produce normal stromas.The results of the weight of production and content of cordycepin according to the statistics were that C.militaris C16 had a significantly higher production than the original strain,and C.militaris C6 had a significantly higher content of cordycepin than the original strain,respectively.

关 键 词：北虫草 紫外诱变 拮抗试验 脂酶同工酶 产量 虫草素 

分 类 号：S567.35[农业科学—中草药栽培]