吡非尼酮对防治大鼠外伤性PVR的疗效观察  

Observations on the Effects of Pirfenidone on Treating Traumatic Proliferative Vitreoretinopathy of Rats

作　　者：刘晏辰[1] 邓爱军[2] 丁敏[1] 刘艳[1] 

机构地区：[1]潍坊医学院眼科学教研室,山东潍坊261053 [2]潍坊医学院附属医院眼科中心

出　　处：《潍坊医学院学报》2013年第1期29-31,34,共3页Acta Academiae Medicinae Weifang

摘　　要：目的观察吡非尼酮经口给药对增值性玻璃体视网膜病变（PVR）的防治作用。方法大鼠静脉血离心后制备成血小板密度为2．5X10^8／ml的血浆。用随机数字法将60只Wistar大鼠随机分成5组，l，2，3组分别为吡非尼酮低浓度组、中浓度组 、高浓度组，4组为模型对照组，5组为空白对照组。建立PvR模型。1，2，3组每天分别给予50rag／kg，150mg／kg，450mg／kg浓度的吡非尼酮灌胃。4组每天给予相同剂量的生理盐水灌胃，正常对照组大鼠不做任何处理。自PVR模型建造术后连续观察眼底28d，并对第28天各组形成的PVR进行分级。玻璃体腔注射后第28天处死动物。结果术后28d，实验各组都有不同程度的PVR表现。术后裂隙灯下连续观察，证实实验组大鼠形成l，2，3级PVR。对各实验纽PVR进行分级结果显示，应用吡非尼酮中、高浓度组PVR分级显著低于模型组（P〈0．05）。结论吡非尼酮经稿给药能够在一定程度上抑制PVR的进展。Objective To investigate the experimental therapeutic effects of pirfenidone by mouth on treating traumatic proliferative vitreoretinopathy. Methods Sixty healthy Wistar rats were randomly divided into normal control group （ 12 rats）, model group （ 12 rats） and PFD （ Pirfenidone ） group （ 36 rats）. The model group and PFD group were established as a vitreoretinopathy. Thirty-six SD rats were divided into PFD micro-dose, PFD moderate-dose and PFD macro-dose group according to the treated concentration of PFD（5Omg/kg, 150mg/kg,450mg/kg）. The rats in PFD group were fed with PFD by stomach perfusion and the rats of normal control received normal saline perfusion of the same w）lume. The changes of eyes were observed after operation by the fundus lens and surgery microscope. All the rats were killed on the 28th day after the operation. Changes of retinal morphology were observed by light microscope. Results PVR degrees in the micro-dose group and model group appeared higher than moderate-dose group and macro-dose group （ P 〉 O. 05 ）. Conclusion Pirfenidone administrated by stomach perfusion is able to inhibit the developing process of PVR.

关 键 词：玻璃体视网膜病变 增生性 视网膜色素上皮细胞 吡非尼酮 灌胃 

分 类 号：R774.12[医药卫生—眼科]