去整合素echistatin对人晶状体上皮细胞增生、黏附、移行的影响  

Effects of echistatin on proliferation, adhestion and migration of human lens epithelial cell in vitro

作　　者：周星[1] 谭少健[1] 粱皓[1] 陈迎迎[1] 李霞[1] 

机构地区：[1]广西医科大学第一附属医院眼科,南宁530021

出　　处：《中华实验眼科杂志》2013年第4期329-333,共5页Chinese Journal Of Experimental Ophthalmology

基　　金：国家自然科学基金项目（81160120）

摘　　要：背景随着白内障手术的广泛开展，后发性白内障（PCO）的发病率逐渐升高，近年来的研究证实，去整合素类药物对PCO有抑制作用。目的探讨去整合素echistatin（Ecs）对人晶状体上皮细胞（LECs）株SRA01／04增生、黏附和移行的影响。方法取对数生长期的SRAOI／04细胞，培养液中加入不同质量浓度的去整合素Ecs（0、2．5、5．0、7．5、10．0、15．0、20．0mg／L）共培养细胞24、48、72h，用MTT法检测药物对细胞增生的影响及药物作用90rain后对细胞黏附的影响；对融合的细胞进行划痕试验，在倒置显微镜下观察并测量划痕宽度，干预培养24h、48h后测量记录划痕愈合情况。结果不同质量浓度去整合素Ecs（2．5、5．0、7．5、10．0、15．0、20．0mg／L）作用24、48、72h后对SRA01／04细胞增生的抑制率分别为2．6％、15．4％、21．2％、34．7％和46．1％、58．2％，6．6％、21．9％、38．2％、50．0％、60．7％、76．9％以及9．8％、29．0％、46．6％、63．4％、69．1％、92．4％。除2．5mg／L组外，各质量浓度组吸光度（A490）值与0mg／L组比较差异均有统计学意义（P〈0．05），且随Ecs作用时间的延长，细胞的A490值逐渐下降，差异有统计学意义（P〈0．05）；Ecs对各质量浓度组SRA01／04细胞的黏附抑制率分别为2．6％、15．0％、26．1％、35．3％、45．2％、54．5％，除2．5mg／L组外，各质量浓度组A490值与0mg／L组比较差异均有统计学意义（P〈O．05）；Ecs作用24h、48h后各质量浓度组SRA01／04细胞的移行距离与0mg／L组相比有不同程度的缩短，差异有统计学意义（P〈0．05），且随Ecs作用时间的延长，细胞移行距离缩短，差异有统计学意义（P〈0．05）。结论去整合素Ecs可抑制人LECs的增生、黏附和移行，其作用呈剂量和时间依赖性，Ecs对PCO的防治可起到积极作用。Background The incidence of posterior capsular opaeifieation （PCO） is increasing with the growing of cataract surgery rate. Recent researches provend that disintegrin has inhibitory effect on PCO, and eehistatin is one of the disintegrin prime families. Objective This study was to investigate the effects of disintegrin and echistatin on proliferation, adhestion and migration in human lens epithelial cells （LECs） line （SRA01/04）. Methods Human LECs line at logarithmic growth phase was used in the study. Cells were cocuhured with medium and different concentrations of echistatin （0, 2.5,5.0, 7.5, 10. 0, 15.0, 20. 0 rag/L） for different time. The proliferative inhibitory rates of LECs were detected by MTT method 24,48 and 72 hours after cultured. Anti-adhesion effect of echistatin were analyzed by the same assay in 90 minutes. Cell scratching test was performed to evaluate the migration ability of LECs. The width of the scratch was recorded in the culture plate covered with cells under an inverted microscope. After being cultured for 24 hours and 48 hours with echistatin, cell migration distances was examined. Results Compared with the 0 mg/L echistatin group,cells proliferation was obviously inhibited. After cultured with 2.5,5.0,7.5,10.0,15.0,20.0 mg/L echistatin, the proliferation inhibitory rate was 2.6% , 15.4% , 21.2%,34.7%,46.1%,58.2% at 24 hours; 6. 6%,21.9%,38.2%,50.0%,60.7%,76.9% at 48 hours and 9.8% ,29.0% ,46.6% , 63.4% , 69.1% , 92.4% at 72 hours, respectively. The absorbance value （ A ） in the 5.0, 7.5,10.0,15.0,20.0 mg/L groups were significantly lower than that in the 0 mg/L group （P〈 0. 05 ）. With theprolongation of acting time of Ecs, the A value of the cells was gradually reduced, with statistically significant difference （P〈0.05）. The adhesion inhibitory rate was 2.6% , 15.0% , 26. 1% , 35.3% ,45.2% and 54.5% in the 2.5,5.0,7.5,10.0,15.0,20. 0 mg/L group, respectively. Compared with the result in the 0 mg/L group, the A value in the 5.0,7.5,10. 0,15. 0,20. 0 mg/L g

关 键 词：后发性白内障 去整合素 晶状体上皮细胞 ECHISTATIN 

分 类 号：R776.1[医药卫生—眼科]