Optimizing rhamnolipid production by Pseudomonas aeruginosa ATCC 9027 grown on waste frying oil using response surface method and batch-fed fermentation  

作　　者：罗致 袁兴中 钟华 曾光明 刘智峰 马小玲 朱雅婷 

机构地区：[1]College of Environmental Science and Engineering, Hunan University [2]Key Laboratory of Environmental Biology and Pollution Control of Ministry of Education (Hunan University)

出　　处：《Journal of Central South University》2013年第4期1015-1021,共7页中南大学学报（英文版）

基　　金：Project(108100) supported by the Key Program for Science and Technology Research of Ministry of Education of China;Projects(50978087, 50908081) supported by the National Natural Science Foundation of China;Project(531107011019) supported by the Hunan University Graduate Education Innovation Program, China;Project(CX2010B157) supported by the Hunan Provincial Innovation Foundation for Postgraduate students, China

摘　　要：Rhamnolipid production by Pseudomonas aeruginosa ATCC 9027 with waste frying oil as sole carbon source was studied using response surface method. Cultures were incubated in shaking flask with temperature, NO3- and Mg2+ concentrations as the variables. Meanwhile, fed-batch fermentation experiments were conducted. The results show that the three variables are closely related to rhamnolipid production. The optimal cultivation conditions are of 6.4 g/L NaNO3 , 3.1 g/L MgSO4 at 32 ℃, with the maximum rhamnolipid production of 6.6 g/L. The results of fed-batch fermentation experiments show that feeding the oil in two batches can enhance rhamnolipid production. The best time interval is 72 h with the maximum rhamnolipid production of 8.5 g/L. The data are potentially useful for mass production of rhamnolipid on oil waste with this bacterium.Rhamnolipid production by Pseudomonas aeruginosa ATCC 9027 with waste frying oil as sole carbon source was studied using response surface method. Cultures were incubated in shaking flask with temperature, NO3- and Mg2＋ concentrations as the variables. Meanwhile, fed-batch fermentation experiments were conducted. The results show that the three variables are closely related to rhamnolipid production. The optimal cultivation conditions are of 6.4 g/L NaNO3, 3.1 g/L MgSO4 at 32℃, with the maximum rhamnolipid production of 6.6 g/L. The results of fed-batch fermentation experiments show that feeding the oil in two batches can enhance rhamnolipid production. The best time interval is 72 h with the maximum rhamnolipid production of 8.5 g/L. The data are potentially useful for mass production of rhamnolipid on oil waste with this bacterium.

关 键 词：Pseudomonas aeruginosa RHAMNOLIPID waste frying oil response surface method FED-BATCH 

分 类 号：TE667[石油与天然气工程—油气加工工程]