FED-BATCH

作品数:22被引量:54H指数:5
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相关作者:刘云王杰葛珍珍余晓斌袁振宏更多>>
相关机构:中国科学院昆明理工大学江南大学更多>>
相关期刊:《Frontiers in Biology》《Science China Chemistry》《中国当代医药》《Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)》更多>>
相关基金:国家自然科学基金河南省科技攻关计划“重大新药创制”科技重大专项更多>>
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基于动态优化的青霉素Fed-batch发酵过程分段操作策略
《化学反应工程与工艺》2023年第6期501-510,共10页伍子奇 吴思辰 翟持 
云南省重大科技项目(202202AG050001);云南省基础研究计划基金(202001AU070048)。
Fed-batch操作因其能有效改善微生物生长环境、延长发酵时间和消除原料及产物抑制而广泛应用于发酵工业中。从动态优化的理论及数值计算两方面,对发酵过程的最优给料参考轨迹进行分析和模拟计算,结果显示,应用基于有限元的正交配置方法...
关键词:开关控制 正交配置 最优给料策略 零阶保持 
Fed-batch培养中补料及接种密度对RABV-G蛋白表达量的影响
《中国当代医药》2022年第36期18-21,共4页宋路萍 邢体坤 王亚萍 李静波 宋彦君 张静静 
“重大新药创制”科技重大专项(2018ZX09736010);河南省重大科技专项(201110310100)。
目的研究流加(Fed-batch)培养中补料及接种密度对狂犬病毒糖蛋白G(RABV-G)蛋白表达量的影响,初步建立RABV-G蛋白在重组中国仓鼠卵巢(CHO)细胞中Fed-batch培养的较优工艺方案。方法复苏培养1支能够稳定表达狂犬病毒RABV-G蛋白的重组CHO细...
关键词:狂犬病病毒 糖蛋白 流加培养 
Constitutive expression of tyrosine phenol‑lyase from Erwinia herbicola in Escherichia coli for L‑DOPA production
《Systems Microbiology and Biomanufacturing》2022年第1期186-192,共7页Mengqing Tang Yajun Bai Tai‑Ping Fan Xiaohui Zheng Yujie Cai 
We thank the National Key Scientifc Instrument and Equipment Development Project of China(2013YQ17052504);the Program for Changjiang Scholars and Innovative Research Team in the University of Ministry of Education of China(IRT_15R55);the seventh group of Hundred-Talent Program of Shanxi Province(2015);The Key Project of Research and Development Plan of Shaanxi(2017ZDCXLSF-01-02-01)for fnancial support.
L-DOPA is a major drug for the treatment of Parkinson’s disease,and microbial enzymatic synthesis is an essential way for its industrial production.The expression of tyrosine phenol-lyase(TPL)derived from Erwinia her...
关键词:L-DOPA Tyrosine phenol-lyase Constitutive promoters Fed-batch mode 
Characterization and application of a recombinant dopa decarboxylase from Harmonia axyridis for the efficient biosynthesis of dopamine
《Chinese Journal of Chemical Engineering》2022年第1期449-456,共8页Siyuan Gao Yuanke Guo Chen Ma Ding Ma Kequan Chen Pingkai Ouyang Xin Wang 
funded by the National Natural Science Foundation of China(21576134 and 21706126);the National Key Research and Development Program(2016YFA0204300)。
Here,a dopa decarboxylase(DDC)from Harmonia axyridis was heterogeneously expressed in Escherichia coli for the efficient biosynthesis of dopamine.For the production of recombinant DDC,the cultivation conditions includ...
关键词:Dopa decarboxylase DOPAMINE Enzyme catalysis Fed-batch bioconversion 
An Approach to Continuous Approximation of Pareto Front Using Geometric Support Vector Regression for Multi-objective Optimization of Fermentation Process被引量:1
《Chinese Journal of Chemical Engineering》2014年第10期1131-1140,共10页吴佳欢 王建林 于涛 赵利强 
Supported by the National Natural Science Foundation of China(20676013,61240047)
The approaches to discrete approximation of Pareto front using multi-objective evolutionary algorithms have the problems of heavy computation burden, long running time and missing Pareto optimal points. In order to ov...
关键词:Continuous approximation of PARETO front GEOMETRIC support vector regression Interactive DECISION-MAKING procedure FED-BATCH FERMENTATION process 
Enhanced Spore Production of <i>Bacillus subtilis</i>Grown in a Chemically Defined Medium被引量:5
《Advances in Microbiology》2014年第8期444-454,共11页S. M. S. Monteiro J. J. Clemente M. J. T. Carrondo A. E. Cunha 
Spores of Bacillus subtilis are being used as probiotics and competitive exclusion agents for animal consumption. Commercial production media often include relatively expensive components of animal origin that are a p...
关键词:Bacillus SUBTILIS SPORES PROBIOTICS Bioreaction FED-BATCH 
Screening of Microalgae for Biodiesel Feedstock
《Advances in Microbiology》2014年第7期365-376,共12页Xi Chen Guoqiang He Zhiping Deng Nan Wang Wei Jiang Sanfeng Chen 
Three heterotrophic microalgae identified as Scenedesmus sp. Y5, Scenedesmus sp. Y7 and Chorellasp. Y9 were isolated and screened from natural water based on biomass yield and lipid productivity. Fatty acids’ composi...
关键词:BIODIESEL SCENEDESMUS CHLORELLA FED-BATCH Fermentation Microalgal Lipid 
Isolation of enriched-yielders and fed-batch production of alkaline protease from the newly isolated <i>Bacillus</i>sp. BHA
《Natural Science》2013年第6期1-9,共9页Bhawana Agarwal Brajesh S. Katiyar 
An alkalophilc and thermophilic Bacillus sp. BHA that produced a thermostable alkaline protease was isolated from decaying protein substrates. The isolate was found to grow in pH range 7 - 11 with an optimum pH 9.0 an...
关键词:Industrial Enzymes Alkaline PROTEASE Feedback REPRESSION Batch Culture FED-BATCH Process 
Optimizing rhamnolipid production by Pseudomonas aeruginosa ATCC 9027 grown on waste frying oil using response surface method and batch-fed fermentation被引量:1
《Journal of Central South University》2013年第4期1015-1021,共7页罗致 袁兴中 钟华 曾光明 刘智峰 马小玲 朱雅婷 
Project(108100) supported by the Key Program for Science and Technology Research of Ministry of Education of China;Projects(50978087, 50908081) supported by the National Natural Science Foundation of China;Project(531107011019) supported by the Hunan University Graduate Education Innovation Program, China;Project(CX2010B157) supported by the Hunan Provincial Innovation Foundation for Postgraduate students, China
Rhamnolipid production by Pseudomonas aeruginosa ATCC 9027 with waste frying oil as sole carbon source was studied using response surface method. Cultures were incubated in shaking flask with temperature, NO3- and Mg2...
关键词:Pseudomonas aeruginosa RHAMNOLIPID waste frying oil response surface method FED-BATCH 
Effects of Fed-batch and Nitrogen-deficient Culture on Lipid Yield of Chlorella sp.
《Agricultural Science & Technology》2012年第12期2453-2456,2460,共5页葛珍珍 王杰 余晓斌 
[Objective] This study was to realize high-density culture of Chlorella sp. as well as the culture with high lipid yield. [Method] Through analyzing the growth curve of Chlorella sp. cells, dextrose consumption curve,...
关键词:Chlorella sp. FED-BATCH Nitrogen-deficient culture Lipid content 
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