Constitutive expression of tyrosine phenol‑lyase from Erwinia herbicola in Escherichia coli for L‑DOPA production  

作　　者：Mengqing Tang Yajun Bai Tai‑Ping Fan Xiaohui Zheng Yujie Cai 

机构地区：[1]The Key Laboratory of Industrial Biotechnology,Ministry of Education,School of Biotechnology,Jiangnan University,No.1800 Lihu Avenue,Wuxi 214122,Jiangsu,China [2]College of Life Sciences,Northwest University,Xi’an 710069,Shaanxi,China [3]Department of Pharmacology,University of Cambridge,Cambridge CB21T,UK

出　　处：《Systems Microbiology and Biomanufacturing》2022年第1期186-192,共7页系统微生物学与生物制造（英文）

基　　金：We thank the National Key Scientifc Instrument and Equipment Development Project of China(2013YQ17052504);the Program for Changjiang Scholars and Innovative Research Team in the University of Ministry of Education of China(IRT_15R55);the seventh group of Hundred-Talent Program of Shanxi Province(2015);The Key Project of Research and Development Plan of Shaanxi(2017ZDCXLSF-01-02-01)for fnancial support.

摘　　要：L-DOPA is a major drug for the treatment of Parkinson’s disease,and microbial enzymatic synthesis is an essential way for its industrial production.The expression of tyrosine phenol-lyase(TPL)derived from Erwinia herbicola under six diferent constitutive promoters in Escherichia coli was studied.The recombinant strain 5 can express TPL well with growth.The optimal medium composition of this strain was as follows:tryptone 18 g/L,yeast extract 28 g/L,glycerol 10 g/L.After the cell culture is completed,the collected cells were used to catalyze the production of L-DOPA,and the concentration can reach 67.9 g/L.

关 键 词：L-DOPA Tyrosine phenol-lyase Constitutive promoters Fed-batch mode 

分 类 号：F42[经济管理—产业经济]