枸杞多糖对顺铂诱导人胚肾细胞凋亡的影响  

Effect of Polysaccharides from Lycium barbarum on Cisplatin-Induced Apoptosis of Human Embryonic Kidney Cells

作　　者：郭培培[1] 高丽萍[1] 冷洪涛[1] 郭卓雨[1] 鲁政[1] 

机构地区：[1]北京联合大学应用文理学院,生物活性物质与功能食品北京市重点实验室,北京100191

出　　处：《食品科学》2013年第7期284-288,共5页Food Science

基　　金：北京联合大学人才强校计划人才资助项目(20111101);北京市人才强教深化计划项目(PHR201107150)

摘　　要：目的:研究枸杞多糖(LBP)对顺铂(CDDP)所致人胚肾细胞(HEK293)凋亡的影响,并探讨其可能机理。方法:体外培养HEK293,四甲基偶氮噻唑蓝(MTT)法观察LBP对CDDP诱导HEK293细胞生长的影响,流式细胞仪检测LBP对CDDP诱导HEK293细胞凋亡率的变化:Western blotting检测LBP对CDDP诱导HEK293细胞凋亡相关基因Bax、Bcl-2蛋白表达的影响。结果:LBP能够拮抗CDDP诱发的细胞毒性,并且随着质量浓度的不同而产生变化。流式细胞仪检测表明随着LBP质量浓度的增加,细胞凋亡率随之减少;Western blotting表明,加入LBP后能够使Bax基因的表达减少而Bcl-2基因的表达增加。结论:LBP对CDDP诱导人胚肾细胞凋亡有抑制的作用,其作用机理可能与其下调Bax及上调Bcl-2蛋白表达有关。This study aimed to explore the effect of polysaccharides from Lycium barbarum （LBP） on the cisplatin-induced apoptosis of human embryonic kidney cells and to investigate its possible mechanism. MTT method was used to observe the effect of LBP and cisplatin on the cell viability of HEK293 cells. CDDP-induced apoptosis was analyzed by flow cytometry and Western blotting was applied to determine the expression of apoptotic gene Bax and anti-apoptosis gene Bcl-2. LBP was able to antagonize cisplatin-induced cytotoxicity in a concentration-dependent manner. Flow cytometry revealed that increasing LBP concentrations led to a decrease in the apoptosis rate and pro-apoptotic Bax gene expression while antiapoptotic gene Bcl-2 expression was enhanced. LBP demonstrated a preventive effect on cisplatin-induced injury in EK293 cells. The mechanism may be related to its capacity of enhancing anti-apoptotic gene Bcl-2 expression and inhibiting proapoptotic gene Bax expression.

关 键 词：顺铂 枸杞多糖 人胚肾细胞 细胞凋亡 

分 类 号：R318.0[医药卫生—生物医学工程]