电堆集-场放大进样非水毛细管电泳法测定苦参中的三种生物碱  

Determination of Three Alkaloids in Sophora flavescens by Nonaqueous Capillary Electrophoresis with Electrostacking-Field-Amplified Sample Injection

作　　者：李利军[1] 孙科[1] 吴美艳[1] 罗应[1] 胡大春[1] 邓春燕[1] 李彦青[1] 

机构地区：[1]广西工学院生物与化学工程系,广西柳州545006

出　　处：《分析科学学报》2013年第2期154-158,共5页Journal of Analytical Science

基　　金：国家自然科学基金(No.20665001);广西科学基金(No.0832062)

摘　　要：建立了电堆集-场放大进样非水毛细管电泳法分离测定苦参中的槐定碱、苦参碱和氧化苦参碱。采用未涂层熔融石英毛细管(50cm×50μm i.d.,有效柱长36cm),紫外检测波长为209nm,运行缓冲溶液为50mmol/L乙酸铵-20%乙腈-0.75%乙酸-55%甲醇,分离电压20kV,电动进样20kV、15s,重力进水柱时间20s时达到最佳的分离效果。在优化条件下,上述三种生物碱均在15min内出峰,峰面积的相对标准偏差(RSD)均小于5%。检出限分别达到2.02μg/L、1.32μg/L和1.01μg/L。An electrostacking-field-amplified sample injection （FASI） method was used for the simultaneous separation and determination of sophoridine, matrine and oxymatrine in Sophora flavescens. The result indicated that a best separation of the three alkaloids was obtained on a uncoated fused silica capillary column（50 cm× 50 μm i. d., effective length 36 cm） by using the background electrolyte containing 50 mmol/L ammonium acetate,20% acetonitrile（by volume）,0. 75% acetic acid （by volume） and 55% methanol（by volume）. The detection wavelength was set at 209 nm, separation voltage was water was 2 the relative 20 kV,sample injection voltage was 20 0 s. Under the optimum conditions,the standard deviations of peak areas 1 kV and injection time was 15 s,and injection time three alkaloids were separated within 15 minutes w ess than sophoridine,matrine and oxymatrine were 2.02 μg/L,1.32 5%. The detection limits （S/N = 3） μg/L and 1.01μg/L,respectively. of th of

关 键 词：电堆集 场放大进样 非水毛细管电泳 槐定碱 苦参碱 氧化苦参碱 

分 类 号：O657.8[理学—分析化学]