微乳毛细管电泳法测定脑心通胶囊中4种有效成分  

Determination of Paeoniflorin,Ferulic Acid,Salvianolic Acid B and Tanshinone ⅡA in Naoxintong Capsule by Microemulsion Electrokinetic Chromatography

作　　者：韦湫阳[1,2] 徐远金[1] 

机构地区：[1]广西大学亚热带农业生物资源保护与利用国家重点实验室,广西南宁530004 [2]广西大学化学化工学院,广西南宁530004

出　　处：《分析科学学报》2013年第2期159-163,共5页Journal of Analytical Science

基　　金：广西自然科学基金(桂科自0832034);广西研究生教育创新计划(T32531)

摘　　要：建立了一种微乳毛细管电泳法同时检测脑心通胶囊中芍药苷、阿魏酸、丹酚酸B和丹参酮ⅡA 4种有效成分的含量。使用未涂层熔融石英毛细管(60cm×75μm i.d.,有效长度50cm)为分离通道,以50mmol/L十二烷基硫酸钠(SDS)-60mmol/LTris(含8.3%正丁醇,1.3%正己烷和15%乙腈,pH=8.8)作电解质,于230nm波长下进行检测。芍药苷、阿魏酸、丹酚酸B和丹参酮ⅡA的质量浓度分别在10～300mg/L、2.5～80mg/L、10～100mg/L和5.0～80mg/L范围与其峰面积呈良好的线性关系,4种物质的检出限分别为1.5mg/L、0.5mg/L、2.0mg/L和1.0mg/L。样品加标回收率在92%～105%之间,相对标准偏差均小于3.2%。方法简便、快速,已用于实际样品的分析。A microemulsion electrokinetic chromatographic （MEEKC） method was developed for the determination of paeoniflorin, ferulie acid,salvianolic acid B and tanshinone 11 A in Naoxintong Capsule. The separation was carried out on an uncoated fused-silica capillary column（60 cm× 75μm,i. d. ,effective length 50 cm）,with 50 mmol/L SDS-60 mmol/L Tris,containing 8.3% n-butanol,1.3% hexane and 15% acetonitrile, pH = 8. 8, as running buffer. Under the optimized conditions, the calibration curves were linear in the range of 10-300 mg/L, 2. 5-80 mg/L, 10-100 mg/L and 5. 0-80 mg/L for paeoniflorin,ferulic acid,salvianolic acid B and tanshinone I] A with the detection limits of 1.5 mg/L, 0.5 mg/L,2.0 mg/L and 1.0 mg/L, respectively. The average recoveries of the four components were between 92.0% and 105% with all relative standard deviations not more than 3. 2%. The proposed 1method was simple,rapid and effective,and could be used for the quality control of Naoxintong capsule.

关 键 词：微乳毛细管电泳 脑心通胶囊 芍药苷 阿魏酸 丹酚酸B 丹参酮ⅡA 

分 类 号：O657.8[理学—分析化学]