聚γ-谷氨酸对气相色谱-质谱检测地衣芽孢杆菌胞内代谢物的影响  

Effect of Poly-γ-glutamate on the Detection of Intracellular Bacillus licheniformis by Gas Chromatography-Mass Spectrometry

作　　者：龙丹[1] 李欣[1] 冀志霞[1,2] 李俊辉[2,3] 祁高富 陈守文[1,2] 

机构地区：[1]华中农业大学农业微生物学国家重点实验室,湖北武汉430070 [2]华中农业大学浦城绿康生物工程研究所,湖北武汉430070 [3]绿康生化股份有限公司,福建浦城353400

出　　处：《分析科学学报》2013年第2期187-190,共4页Journal of Analytical Science

基　　金：国家自然科学基金(No.31170046)

摘　　要：通过添加外源聚γ-谷氨酸(γ-PGA),利用气相色谱-质谱法(GC-MS)评估了γ-PGA对地衣芽孢杆菌胞内代谢物检测的影响。结果表明:终浓度为10g/L的γ-PGA会使除谷氨酸外的氨基酸、有机酸和糖类标准品的检测峰面积明显降低;另外,终浓度为10g/L的γ-PGA对分析地衣芽孢杆菌胞内代谢物中的谷氨酸、葡萄糖和6-磷酸葡萄糖(G6P)的影响较为显著。因此,在利用GC-MS定量分析地衣芽孢杆菌的胞内代谢物前,应将γ-PGA和细胞分离。In this work, we assessed the effect of exogenous poly-γ-glutamate on the detection of intracellular metabolites in Bacillus licheniformis by gas chromatography-mass spectrometry（GC-MS）. The results indicated that 10 g/L γ-PGA added in standard solutions obviously decreased the peak areas of amino acids,organic acids and sugars, except glutamate. For the intracellular metabolites of Bacillus licheni f ormis , the phosphate were al before quantifying effects of γ-PGA on the detection of intracellular glutamate, glucose and glucose 6- so extremely remarkable. Therefore,it is necessary to isolate γ-PGA from the broth the intracellular metabolites by GC-MS.

关 键 词：聚Γ-谷氨酸 气相色谱-质谱 地衣芽孢杆菌 代谢物 样品预处理 

分 类 号：O657.63[理学—分析化学]