转化生长因子β1对A549细胞晚期糖基化终产物受体与细胞外基质表达的影响  

Expression of RAGE and extracellular matrix induced by transforming growth factor beta 1 in A549 cells

作　　者：丁辉[1] 冯艳[1] 陈如华[1] 

机构地区：[1]宜兴市人民医院呼吸内科,214200

出　　处：《国际呼吸杂志》2013年第6期415-419,共5页International Journal of Respiration

摘　　要：目的研究转化生长因子Bl（transforminggrowthfactorbeta1，TGF-β1）对人A549细胞的晚期糖基化终产物受体（receptorforadvancedglycationend—products，RAGE）和胶原-I、a-平滑肌肌动蛋白（a-smoothmuscleactin，d—SMA）表达的作用。方法体外培养人A549细胞，以不加TGF-[31刺激为对照组，不同终浓度TGF-β1（2μg／L、5,ag／L、10／μg／L）处理A549细胞为实验组，RT-PCR和Westernblotting法检测TGF-β1刺激12h、24h、36h后对照组和实验组RAGEmRNA和RAGE、胶原-I及a—SMA的蛋白表达。结果①随着TGF-β1，浓度的增加，刺激时间的延长，A549细胞的RAGEmRNA和蛋白的表达逐渐降低，呈时间-浓度依赖关系。②与对照组相比，A549在TGF-β1（5μg／L）刺激24h后，RAGEmRNA的表达明显降低（0．387±0．088VS1．345±0．132，P〈0．01）。RAGE蛋白表达较对照组明显降低（O．174±0．061VS1．229±0．112，P〈0．01）。③TGF-β1（5μg／L）刺激24h后，A549的胶原-I及d—SMA蛋白表达（0．916±0．071和0．899±0．022）分别较对照组（0．295±0．041和0．134±0．028）升高3．1和6．7倍，差异有统计学意义（P〈O．01）。④相关分析显示，A549的RAGE蛋白表达与胶原-I（r=-0．843，P〈0．05）、n—SMA蛋白的表达（r=-0．897，P〈0．05）呈负相关。结论TGF-β3。下调A549细胞RAGE的mRNA和蛋白表达，与胶原-I及a—SMA蛋白表达上调呈负相关，提示RAGE的表达下调可能与肺纤维化的发生和发展有关。Objective To investigate the expressions of receptor for advanced glycation endproducts （RAGE）, collagen-I , and asmooth muscle actin （a-SMA） in human A549 cells induced by transforming growth factor beta 1 （TGF-β1 ）. Methods With no TGF-β1-stimulated cells for the control group, human A549 cells were cultured in vitro. Cultured cells were exposed to TGF-β1with different final concentration （2 /μg/L,5 g/L,10 μg/L） for different time （12 h,24 h,36 h）. RAGE mRNA and protein, collagen- I and a-SMA in cells were detected at different points after the treatment of by RT-PCR and Western blotting. Results @TGF-β1reduced RAGE mRNA and protein expressions in a dose- and time-dependent manner. @Compared with the control group, RAGE mRNA （0. 387±0. 088 vs 1. 345±0. 132） and protein expressions （0. 174±0. 061 vs 1. 229±0. 112） were significantly reduced （ P 〈 0.01） in cells exposed to TGF-β1 （5 /μg/L） for 24 hours. @The collagenI and a-SMA protein expressions increased after stimulation for 24 hours in 5 μg/L TGF-β1 group. At that point, collagen- β1 and a-SMA protein expressions were 3.1 and 6.7 times of the control group （ P 〈0.01）. @RAGE expression had a negative correlation with collagen-I （r =--0. 843, P 〈0.05） and a-SMA protein expression （r = -0. 897, P 〈0.05）. Conclusions The reduced RAGE, which is negatively correlated with expressions of collagen- I and a-SMA proteins in A549 cells exposed to TGF-β1, may be related to the development and progress of pulmonary fibrosis.

关 键 词：TRANSFORMING growth factor beta 1 Receptor for advanced glycation end—products d—smooth muscle ACTIN Collagen-I 

分 类 号：R285.5[医药卫生—中药学]