管腔内局部照射诱发急性放射性食管损伤动物模型的构建及其应用  

Development of a rat model of intraluminal local radiation-induced acute radioactive esophageal injury

作　　者：仝甲钊[1] 曲波[1] 王耀明[2] 金世柱[1] 崔亚利[3] 辛然[4] 王蓓蓓[1] 姜海燕[1] 

机构地区：[1]哈尔滨医科大学附属第二医院消化内科,黑龙江省哈尔滨市150086 [2]黑龙江省医院放射科,黑龙江省哈尔滨市150036 [3]哈尔滨医科大学附属第三医院放射科,黑龙江省哈尔滨市150086 [4]哈尔滨医科大学附属第二医院重症医学科,黑龙江省哈尔滨市150086

出　　处：《世界华人消化杂志》2013年第9期791-797,共7页World Chinese Journal of Digestology

基　　金：哈尔滨市科委青年科技创新人才基金资助项目;No.2018rfqys103~~

摘　　要：目的:构建急性放射性食管损伤动物模型,并进行临床应用方法学探讨.方法:应用不同初始活度的125I粒子链于SD大鼠食管管腔内进行局部照射,分别于照射3、5、7d后处死大鼠,取全长食管组织做石蜡包埋、切片、HE染色,与空白组对照,进行病理学分析.观察初始活度0.8mGi125I粒子链食管腔内照射后2wk内大鼠生存状况及其7d组在1wk、2wk时大鼠全长食管组织的病理变化.结果:初始活度在0.6mGi以上的5颗125I粒子组成的粒子链对SD大鼠行食管腔内局部照射5d以上可诱发放射性食管损伤,随粒子活度的增加,放射性食管损伤逐渐加重.术后,125I粒子链可方便取出,不会对大鼠食管进一步损伤,且经125I粒子链食管腔内照射后SD大鼠可逐渐恢复饮食,2wk后大鼠食管放射性损伤有修复的趋势.结论:食管管腔内应用初始活度0.6mGi以上的5颗125I粒子组成的粒子链接近零距离持续照射5d以上能够引起放射性食管损伤.食管腔内应用低能核素125I粒子链近距离放疗或许可为临床姑息治疗晚期食管癌提供方法学借鉴.AIM： To develop a rat model of acute radioac-tive esophageal injury by local irradiation of the esophagus with 125I seed chain and to explore its clinical application. METHODS： 125I seed chain was used to locally irradiate the esophagus of SD rats. The rats were killed on days 3, 5 and 7 after irradiation to take the full-length esophageal tissue. Tissue samples were embedded in paraffin, sectioned, and subjected to HE staining for pathological analysis. Pathological changes in the full-length esophageal tissue at one week and two weeks and living status at two weeks after intraluminal irradiation with 0.8 mGi 125 I seed chain （initial activity 0.8 mGi） were observed. RESULTS： The chain of five 125I seeds, with an initial activity of 0.6 mGi, could induce ra-dioactive esophageal injury by intraluminally irradiating the esophagus for 5 d. Radioactive esophageal injury increased gradually with the increase in particle activity. 125 I seed chain could be easily taken out postoperatively to avoid further esophageal injury. SD rats could gradu-ally resume eating after intraluminal irradiation with 125I seed chain, and the injury tended to be repaired in two weeks. CONCLUSION： The chain of five 125I seeds, with an initial activity of 0.6 mGi, can induce ra-dioactive esophageal injury by intraluminally irradiating the rat esophagus for five days. Intraluminal brachytherapy with low-energy radionuclide 125I seed chain may provide a clinical option for treatment of advanced esophageal cancer.

关 键 词：放射性食管损伤 125I粒子 晚期食管癌 短距离放射疗法 动物模型 

分 类 号：R735.1[医药卫生—肿瘤]