KAP-1在胰腺癌中的表达及临床意义  

Clinical significance of expression of KAP-1 in pancreatic carcinoma

作　　者：江建新[1] 詹磊[1] 黄洋[1] 何燕浙[1] 孙诚谊[1] 

机构地区：[1]贵阳医学院附属医院肝胆外科,贵州省贵阳市550001

出　　处：《世界华人消化杂志》2013年第9期829-834,共6页World Chinese Journal of Digestology

基　　金：国家自然科学基金资助项目;No.81160311;贵州省科教青年英才培养工程基金资助项目;No.黔省专合字[2012]177号;贵州省科技厅2011年度社会攻关计划基金资助项目;No.黔科合SY字[2011]3007;贵州省肝胰疾病研究科技创新人才团队基金资助项目;No.黔科合人才团队[2010]4010~~

摘　　要：目的:研究KAP-1在胰腺癌组织和细胞株中的表达及意义.方法:采用免疫组织化学方法检测46例胰腺癌标本(高分化15例,中分化17例,低分化14例)及9例正常胰腺标本中的KAP-1表达;采用RT-qPCR和Western blot的方法检测8对胰腺癌组织和配对癌旁组织标本中KAP-1的mRNA和蛋白质水平;采用RT-qPCR和Wsetern blot检测胰腺癌细胞株BxPC3、PANC-1、AsPC-1、SW1990、MIAPaCa-2、CFPAC-1、Capan-1和Capan-2中KAP-1mRNA和蛋白的表达水平.结果:免疫组织化学结果显示KAP-1在胰腺癌组织中阳性表达率为45.6%(21/46例),正常胰腺组织中阳性表达率为11.1%(1/9);在低分化胰腺癌组织中阳性表达率为78.6%(11/14),中分化胰腺癌组织为47.1%(8/17),高分化胰腺癌组织为13.3%(2/15);RT-qPCR和Western blot显示在胰腺癌组织中KAP-1的mRNA和蛋白质水平较癌旁正常胰腺组织高,KAP-1的mRNA水平在Panc-1中最高,在BXPC-3和CFPAC-1中较高,在SW1990、Capan-1和MIAPaCa-2中较低,在AsPC-1和Capan-2中最低.KAP-1蛋白质水平在低分化胰腺癌细胞株MIAPaCa-2和Panc-1中高,来源于肝转移的胰腺癌细胞系CFPAC-1中较高,其余细胞株中不表达.结论:KAP-1在胰腺癌组织中高表达,正常胰腺组织中几乎不表达,其表达与胰腺癌细胞分化相关;KAP-1可能在胰腺癌发生发展中发挥重要作用.AIM： To investigate the expression of KAP-1 in pancreatic carcinoma. METHODS： The expression of KAP-1 protein in 46 pancreatic cancer specimens （including 15 cases of highly differentiated cancer, 17 cases of moderately differentiated cancer, and 14 cases of poorly differentiated cancer） and 9 normal pancreas specimens was detected by immuno-histochemistry. The mRNA and protein expression of KAP-1 in 8 cases of pancreatic cancer and matched tumor-adjacent pancreatic tissue and in pancreatic carcinoma cell lines （BxPC3, CA- PAN-1, PANC-1, AsPC-1, SW1990, MiaPaCa-2 and CFPAC-1） was also detected by RT-qPCR and Western blot.RESULTS： The positive rate of KAP-1 expression was 45.6% （21/46） in pancreatic cancer and 11.1% （1/9） in normal pancreatic tissue, and was 78.6% （11/14） in poorly differentiated pancreatic cancer, 47.1% （8/17） in moderately differentiated pancre-atic cancer, and 13.3% （2/15） in highly differenti-ated pancreatic cancer. The mRNA and protein expression of KAP-1 was higher in pancreatic cancer than in matched tumor-adjacent pancreatic tissue. The mRNA expression of KAP-1 was high-est in poorly differentiated pancreatic cancer line Panc-1, higher in BXPC-3 and CFPAC-1, lower in SW1990, Capan-1 and MIAPaCa-2, and lowest in AsPC-1 and Capan-2. The protein expression of KAP-1 was highest in poorly differentiated pancreatic cancer line MIAPaCa-2 and Panc-1, higher in CFPAC-1 which is derived from liver metas-tases of pancreatic cancer cell, and was undetectable in other cell lines.CONCLUSION： The expression of KAP-1 in human pancreatic cancer tissue is significantly higher than that in normal pancreatic tissue, and KAP-1 expression is involved in pancreatic cancer cell differentiation. KAP-1 may play an important role in the development of pancreatic cancer.

关 键 词：胰腺癌 KAP-1 分化 

分 类 号：R735.9[医药卫生—肿瘤]