胰岛素样生长因子-1诱导新生小鼠海马源性神经干细胞增殖分化的研究  

Effects of insulin - like growth factor - 1 on the proliferation and differentiation of cultured neural stem cells in vitro

作　　者：朱裕华[1] 胡安康[1] 陈仁金[1] 吴连连[2] 袁红花[2] 朱孝荣[1] 

机构地区：[1]徐州医学院实验动物中心,江苏徐州221002 [2]徐州医学院神经生物学教研室

出　　处：《徐州医学院学报》2013年第2期76-79,共4页Acta Academiae Medicinae Xuzhou

基　　金：江苏省自然科学基金（BK2010180）;徐州市社会发展科学基金（XM09B116）;徐州医学院院长基金（2010KJZ10）

摘　　要：目的探讨胰岛素样生长因子-1（IGF-1）对新生小鼠海马源性神经干细胞（NSCs）增殖和分化的影响。方法通过取新生C57BL／6J小鼠海马组织，机械分离，体外培养NSCs，特异性标志蛋白Nestin免疫荧光鉴定。在细胞培养液中加入100μg／L的IGF-1记为IGF-1组，同时设立正常细胞对照组。CCK-8试剂盒检测细胞增殖情况，GFAP、133Tubulin细胞免疫化学检测细胞分化情况。结果体外成功培养NSCs，特异性蛋白Nes-tin具有表达。IGF-1组NSCs较对照组增殖显著，且IGF-1组细胞具有显著的迁移和分化能力。细胞免疫荧光鉴定，IGF-1可促进细胞分化为星形胶质细胞和神经元（P〈0．05）。结论IGF-1能够促进体外培养的新生小鼠海马源性NSCs增殖，并诱导NSCs向星形胶质细胞和神经元分化。Objective To detect the effects of insulin - like growth factor - 1 （ IGF - 1 ） on the proliferation and dif- ferentiation of cultured hippoeampal neural stem cells （NSCs） of newborn mouse. Methods NSCs were cultured in vitro through collecting the hippocampus of newborn C57BL/6J mouse and medical separation and then which were detected with specific protein Nestin immunofluorescent staining. The cell culture medium was added 100 μg/L of IGF - 1 as IGF - 1 group and the culture medium with no IGF - 1 as control group. Cells proliferation was detected by CCK - 8 kit and cells differentiation was detected by GFAP and 133 tubulin immunofluorescent staining. Results Successfully cultured NSCs in vitro, and specific protein Nestin had expression. IGF - 1 group cells had more obvious proliferation, migration and differentiation compared with the control group. Immunoiluorescent identification showed that IGF - 1 could promote cells to differentiate into astrocytes and neuron （ P 〈 0.05 ）. Conclusion IGF - 1 can promote the proliferation of hipp- ocampal NSCs of newborn mouse in vitro, and induce the differentiation of NSCs to astrocytes and neuron.

关 键 词：胰岛素样生长因子-1 神经干细胞 增殖和分化 小鼠 

分 类 号：R-332[医药卫生]