人参皂苷Rb1对睡眠剥夺大鼠学习记忆及脑内生长抑素表达的影响  

Effects of ginsenosides Rb1 on learning and memory and expression of somatostatin in sleep deprivation rats

作　　者：董静尹[1] 王俊波[1] 方洁[1] 袁张根[1] 陆柯洁[1] 金怡[1] 曾玲晖[1] 

机构地区：[1]浙江大学城市学院,浙江杭州310015

出　　处：《浙江大学学报（医学版）》2013年第2期197-204,共8页Journal of Zhejiang University(Medical Sciences)

基　　金：浙江大学城市学院大学生科研项目(X2011562098);浙江省教育厅科研基金项目(Y200909882);浙江省自然科学基金项目(Y2100417)

摘　　要：目的:研究人参皂苷Rb1(ginsenosides Rb1,GSRb1)对睡眠剥夺(sleep deprivation,SD)大鼠学习记忆与海马及额叶生长抑素(somatostatin,SS)表达的影响。方法:大鼠随机分为睡眠剥夺2 d组、4 d组、6 d组和未睡眠剥夺(SD 0 d)组,每组再分为GSRb1用药组和生理盐水对照组,共8组。采用改良小平台水环境法制作SD大鼠模型。造模前大鼠分别腹腔内注射GSRb1 30 mg/(kg.d)或生理盐水7 d。睡眠剥夺2 d、4 d和6 d后采用Morris迷宫检测各组大鼠学习记忆能力,免疫组织化学法结合图像分析脑内SS阳性神经元数量及SS平均光密度。结果:与SD0d组大鼠比较,SD各组大鼠游泳平均速度减慢(P<0.01),逃避潜伏期明显延长(P<0.01);海马CA4&DG区SS表达下降(P<0.05);SD 2 d组大鼠额叶SS表达无明显变化(P>0.05),而SD 4 d、6 d组SS表达减少明显(P<0.05)。与生理盐水对照组比较,SD组同时段的GSRb1用药组大鼠游泳平均速度显著加快(P<0.05),逃避潜伏期明显缩短(P<0.05),用药组各时段海马内SS阳性神经元数量均明显增多,SS表达增强(P<0.05)。结论:人参皂苷Rb1能显著改善SD大鼠认知功能,该作用可能与增加海马内SS密切相关。Objective： To determine the effects of ginsenosides Rbl （ GSRbl ） on learning and memory and expression of somatostatin （ SS ） in the hippocampus and the frontal cortex in rat model of sleep deprivation （SD）. Methods： Rats were randomized into groups of SD 2 d, SD 4 d, SD 6 d, and SD 0 d, while each group was sub-divided into GSRbl group and normal saline （ NS ） sub-groups. Rats were intraperitoneal administered with 30 mg/（ kg d） of GSRbl or NS for 7 d,then the learning and memory abilities were examined by measuring average swimming speed and mean escape latency using Morris maze. Expression of somatostatin was detected with immunohistochemical method and image analysis in the hippocampus and the frontal cortex. Results： Compared with SD 0 d rats, SD rats exhibited significant decrease in the average swimming speed and increase in the escape latency （ P 〈 0. O1 ）. The expression of somatostatin in the hippocampus was decreased significantly in SD 2 d, SD 4 d and SD 6 d rats （ P 〈 0. 05 ）. However, decrease was only observed in SD 4 d and SD 6 d rats in the frontal cortex （P 〈 0. 05）. Parallel comparison between NS control and GSRbl treated rats demonstrated that rats treated with GSRbl in each subgroup exhibited faster swimming speed and shorter escape latency （P 〈 0.05 ）. Meanwhile, the expression of somatostatin was increased in SD 2 d, SD 4 d and SD 6 d rats in the hippocampus and in SD 4 d and SD 6 d rats in the frontal cortex （P 〈 0.05 ）, respectively. Conclusion： GSRbl enhances the expression of somatostatin in sleep deprivation rats and subsequently may improve learning and memory abilities of rats.

关 键 词：人参皂甙 治疗应用 生长抑素 学习障碍 药物疗法 记忆障碍 药物疗法 迷宫学习 海马 细胞学 额叶 细胞学 神经元 细胞学 睡眠剥夺 

分 类 号：R285.5[医药卫生—中药学]