bFGF对实验性近视眼动脉血液动力学的影响  

BFGF ON OPHTHALMIC ARTERY BLOOD DYNAMICS IN EXPERIMENTAL MYOPIA

作　　者：格日勒图 王中颖 伟伟 张玉凤 

机构地区：[1]内蒙古自治区人民医院眼科,内蒙古呼和浩特010017

出　　处：《内蒙古医学院学报》2012年第S5期733-736,共4页Acta Academiae Medicinae Neimongol

摘　　要：目的:研究bFGF对兔眼睑缝合法诱导的FDM眼动脉血液动力学的影响,从而探讨bFGF在近视眼的发病机制中的作用。方法:单纯眼睑缝合法建立近视动物模型。取14日龄幼兔45只,分A、B、C三组,每组15只。A组:单纯缝合眼睑;B组:缝合眼睑+结膜下注射bFGF+PBS;C组:缝合眼睑+结膜下注射PBS.均以右眼为实验眼,左眼为对照眼。第60d,分别测量双眼屈光状态、眼周长度、眼动脉阻力及眼动脉收缩期和舒张期血流流速。结果:A、C两组眼睑缝合60d后,与对侧眼相比诱导出相对近视,眼轴相对延长;而B组屈光度和眼轴长度双眼间差异无显著性。各组两眼间的眼动脉阻力及血流流速差异有显著性(P<0.05);就实验眼而言A、C两组间比较差异无显著性(P>0.05),而B组与A、C两组间差异有极显著性(P<0.01)。结论:眼睑缝合可引起幼兔明显的轴性近视,伴随轴性近视出现眼动脉阻力增加、眼动脉血流流速减慢现象,而bFGF可有效减低眼动脉阻力、增加眼动脉血流流速。Objective：To investigate the effect of bFGF in pathological mechanism of myopia by observing ophthalmic artery blood dynamics in cultured rabbit with eyelid sutureinduced form deprived myopia. Methods： Form deprivation method was applied to establish the animal models of myopia by suturation of right eye-lid. Fourteen-day of neonatal rabbits were divided into group A, B andC：in group A（n= 15）,no drug was used, while group B（n= 15） received a serious of subconjunctival injection of bFGF ＋ PBS ＋ insuline and group C （ n = 15 ） received PBS ＋ insuline with monocular deprivation （MD）. Respectively, the left eyes were employed as control groups. 60 days later, data including refractive state, axial length （AL）, ophthalmic artery resistance and ophthalmic artery blood stream velocity were obtained and analyzed. Results：Compared with normal control eyes, experimental eyes had obvious myopia after eyelid suture-inducing for 60 days in group A and group C. Yet these did not show any difference in group B. Each group in right and left eyes, Ophthalmic artery resistance and ophthalmic artery blood stream velocity flow were showed a significant difference. Speaking form deprived eyes, above targets did not show any difference between group A and group C,but comparison group B with group A and group C had notable significant differences. Conclusion： suturation eye-lid can induce axial myopia, higher ophthalmic artery resistance and slower ophthalmic artery blood stream velocity flow in rabbits. While bFGF can block these changes.

关 键 词：碱性成纤维细胞生长因子 实验性近视 超声 血液动力学 

分 类 号：R778.11[医药卫生—眼科] R339.14[医药卫生—临床医学]