非限制翻译后修饰鉴定方法的研究进展  

The Research and Progress of Unrestricted Post-Translational Modifications Search Based on Tandem Mass Spectrometry

作　　者：张成普[1] 李宁[1] 马洁[1] 吴松锋[1] 朱云平[1] 

机构地区：[1]军事医学科学院放射与辐射医学研究所,北京蛋白质组研究中心,蛋白质组学国家重点实验室,北京102206

出　　处：《生物化学与生物物理进展》2013年第4期309-318,共10页Progress In Biochemistry and Biophysics

基　　金：国家重点基础研究发展计划(973)(2011CB910601,2010CB912700);国家高技术研究发展计划(863)(2012AA020409,2012AA020201);国家自然科学基金(21105121)资助项目~~

摘　　要：蛋白质翻译后修饰在真核生物细胞内广泛存在,对蛋白质的结构和功能有着十分重要的影响.串联质谱技术的快速发展为翻译后修饰鉴定提供了高通量、高灵敏度和高分辨率的分析平台,但传统搜索引擎鉴定修饰的方法无法满足数据分析的需求,非限制翻译后修饰鉴定已成为目前蛋白质组修饰分析的重要手段之一.非限制翻译后修饰鉴定不需要在分析前指定修饰类型,可以直接从样品中找出大量已知或未知的修饰,对提高质谱图谱解析率以及揭示蛋白质的生物学功能具有十分重要的意义.本文首先介绍了非限制翻译后修饰鉴定的定义和发展历程,然后从序列匹配和谱图匹配两个方面详细综述了目前非限制翻译后修饰鉴定的主流算法,分析了非限制翻译后修饰鉴定的质量控制问题,最后结合非限制翻译后修饰鉴定的实际应用讨论了修饰鉴定算法的不足和发展方向.Protein post-translational modifications（PTM） are widespread in eukaryotic cells and have a significant influence on the structure and function of proteins.The rapid development of tandem mass spectrometry（MS/MS） has provided a sensitive and accurate platform in high throughput PTM identification.However,the traditional database search engines could not meet the needs of modification data analysis,which has made the unrestricted modification search become one of the major methods/strategies to identify modifications in proteomics.Without requirement to specify the type of modifications in advance,unrestricted modification search can detect a large number of known and unanticipated modifications from the samples,which is of great significance to improve the identification rate of tandem mass spectra and reveal the biological function of proteins.In this paper,we first described the definition and development of unrestricted modification search.Then we discussed the algorithms of unrestricted PTM identification based on two major approaches,sequence matching and spectral matching,as well as the quality control of modification identification.Finally,we summarized several applications of unrestricted PTM identification,and the challenges and strategies discussed here could benefit the future research.

关 键 词：串联质谱 蛋白质翻译后修饰 生物信息学 蛋白质组学 

分 类 号：Q51[生物学—生物化学] Q811.4