青岛地区人乳头状瘤病毒感染亚型与肺鳞状细胞癌的相关性  

The correlation between HPV infection subtype and lung squamous cell carcinoma in Qingdao region

作　　者：高志棣[1] 邵纬[1] 李黎[2] 马晓业[1] 孙玉萍[3] 

机构地区：[1]青岛大学医学院附属海慈医院,山东青岛266033 [2]青岛大学医学院第二附属医院,山东青岛266042 [3]山东大学附属济南市中心医院,山东济南250012

出　　处：《癌变．畸变．突变》2013年第2期111-114,共4页Carcinogenesis,Teratogenesis & Mutagenesis

基　　金：青岛市2011年度医药科研指导计划项目(2010-WSZD026)

摘　　要：目的:检测青岛地区居民肺鳞状细胞癌(squamous cell carcinoma,SCC)组织人乳头状瘤病毒(human papillomavirus,HPV)感染亚型与肺SCC的相关性。方法:收集肺SCC石蜡病理切片196例,提取癌组织和癌旁组织DNA,应用PCR及斑点杂交技术进行HPV基因检测,并对HPV亚型分布及其与肺SCC分级的关系进行分析。结果:在196例标本中,114例(58.16%)存在不同亚型的HPV感染,较癌旁组织14.80%(29/196)明显增高(P<0.05)。主要为HPV6(48/196,24.49%)、HPV16(61/196,31.12%)、HPV18(4/196,2.04%)及HPV58(1/196,0.51%)4型,HPV6阳性主要存在于低度恶性鳞癌中,HPV16阳性主要发生在中高度恶性鳞癌中。吸烟(92.98%)和男性(70.18%)与肺鳞癌HPV感染阳性率正相关(P<0.05),低龄(7.89%)与HPV感染负相关(P<0.05)。结论:HPV感染与肺SCC的形成密切相关,低危型HPV6感染在肺鳞癌发生中的作用值得进一步研究。OBJECTIVE： To find out the prevalence and subtype distribution of human papillomavirus （HPV） among lung squamous cell carcinomas （SCC）. METHODS：We collected and examined 196 paraffin sections of lung SCC in Qingdao patients for the presence of HPV with polymerase chain reaction （PCR） and dot hybridization. RESULTS：We found that 58.16% of （114/196） all samples were positive for HPV. The four main types were HPV6 （48/196,24.49%）,HPV16 （61/196,31.12%）,HPV18 （4/196,2.04%） and HPV58 （1/196,0.51%）. HPV6 mainly existed in the low-grade malignant squamous carcinoma,while HPV16 was mainly found in highly malignant squamous carcinoma. There were correlations between smoking （92.98%）,male gender （70.18%） and HPV positive rate in lung squamous carcinoma. CONCLUSION：There was an obvious relationship between the HPV subtype and the lung SCC. The study provided a theoretical basis for the preventive treatment of HPV vaccine on lung SCC.

关 键 词：人乳头状瘤病毒 聚合酶链反应 肺恶性肿瘤 鳞状上皮细胞癌 

分 类 号：R730.231[医药卫生—肿瘤]